Arthur H and Isabel Bunker Associate Professor of Medicine (Hematology) and Associate Professor of Pathology
Chief, Section of Hematology
Director, DeLuca Center for Innovation in Hematology Research, Yale Cancer Center
Assistant Medical Director CRSL, Yale Cancer Center

Education & Training

Fellow
Yale University School of Medicine (2006)
Resident
Yale-New Haven Hospital (2002)
Postdoctoral Associate
Childrens Hospital Los Angeles (2000)
Dr Med
Eberhard-Karls-Universitat Tubingen (1997)
Resident
Eberhard-Karls-University (1997)
MD
Eberhard-Karls-Universitat Tubingen (1995)

Languages Spoken

English
Deutsch (German)
Italiano (Italian)
Español (Spanish)

Additional Information

Honors & Recognitions

Professional Service

Publications

Editorial: Molecular advances make the day in myeloid diseases
Halene S. Editorial: Molecular advances make the day in myeloid diseases Current Opinion In Hematology 2023, 30: 29. PMID: 36752625, DOI: 10.1097/moh.0000000000000751.
High-plex protein and whole transcriptome co-mapping at cellular resolution with spatial CITE-seq
Liu Y, DiStasio M, Su G, Asashima H, Enninful A, Qin X, Deng Y, Nam J, Gao F, Bordignon P, Cassano M, Tomayko M, Xu M, Halene S, Craft J, Hafler D, Fan R. High-plex protein and whole transcriptome co-mapping at cellular resolution with spatial CITE-seq Nature Biotechnology 2023, 1-5. PMID: 36823353, DOI: 10.1038/s41587-023-01676-0.
Recruited monocytes/macrophages drive pulmonary neutrophilic inflammation and irreversible lung tissue remodeling in cystic fibrosis
Öz H, Cheng E, Di Pietro C, Tebaldi T, Biancon G, Zeiss C, Zhang P, Huang P, Esquibies S, Britto C, Schupp J, Murray T, Halene S, Krause D, Egan M, Bruscia E. Recruited monocytes/macrophages drive pulmonary neutrophilic inflammation and irreversible lung tissue remodeling in cystic fibrosis Cell Reports 2022, 41: 111797. PMID: 36516754, DOI: 10.1016/j.celrep.2022.111797.
NIH SenNet Consortium to map senescent cells throughout the human lifespan to understand physiological health
Lee P, Benz C, Blood P, Börner K, Campisi J, Chen F, Daldrup-Link H, De Jager P, Ding L, Duncan F, Eickelberg O, Fan R, Finkel T, Furman D, Garovic V, Gehlenborg N, Glass C, Heckenbach I, Joseph Z, Katiyar P, Kim S, Königshoff M, Kuchel G, Lee H, Lee J, Ma J, Ma Q, Melov S, Metis K, Mora A, Musi N, Neretti N, Passos J, Rahman I, Rivera-Mulia J, Robson P, Rojas M, Roy A, Scheibye-Knudsen M, Schilling B, Shi P, Silverstein J, Suryadevara V, Xie J, Wang J, Wong A, Niedernhofer L, Wang S, Anvari H, Balough J, Benz C, Bons J, Brenerman B, Evans W, Gerencser A, Gregory H, Hansen M, Justice J, Kapahi P, Murad N, O’Broin A, Pavone M, Powell M, Scott G, Shanes E, Shankaran M, Verdin E, Winer D, Wu F, Adams A, Blood P, Bueckle A, Cao-Berg I, Chen H, Davis M, Filus S, Hao Y, Hartman A, Hasanaj E, Helfer J, Herr B, Joseph Z, Molla G, Mou G, Puerto J, Quardokus E, Ropelewski A, Ruffalo M, Satija R, Schwenk M, Scibek R, Shirey W, Sibilla M, Welling J, Yuan Z, Bonneau R, Christiano A, Izar B, Menon V, Owens D, Phatnani H, Smith C, Suh Y, Teich A, Bekker V, Chan C, Coutavas E, Hartwig M, Ji Z, Nixon A, Dou Z, Rajagopal J, Slavov N, Holmes D, Jurk D, Kirkland J, Lagnado A, Tchkonia T, Abraham K, Dibattista A, Fridell Y, Howcroft T, Jhappan C, Montes V, Prabhudas M, Resat H, Taylor V, Kumar M, Suryadevara V, Cigarroa F, Cohn R, Cortes T, Courtois E, Chuang J, Davé M, Domanskyi S, Enninga E, Eryilmaz G, Espinoza S, Gelfond J, Kirkland J, Kuchel G, Kuo C, Lehman J, Aguayo-Mazzucato C, Meves A, Rani M, Sanders S, Thibodeau A, Tullius S, Ucar D, White B, Wu Q, Xu M, Yamaguchi S, Assarzadegan N, Cho C, Hwang I, Hwang Y, Xi J, Adeyi O, Aliferis C, Bartolomucci A, Dong X, DuFresne-To M, Ikramuddin S, Johnson S, Nelson A, Niedernhofer L, Revelo X, Trevilla-Garcia C, Sedivy J, Thompson E, Robbins P, Wang J, Aird K, Alder J, Beaulieu D, Bueno M, Calyeca J, Chamucero-Millaris J, Chan S, Chung D, Corbett A, Gorbunova V, Gowdy K, Gurkar A, Horowitz J, Hu Q, Kaur G, Khaliullin T, Lafyatis R, Lanna S, Li D, Ma A, Morris A, Muthumalage T, Peters V, Pryhuber G, Reader B, Rosas L, Sembrat J, Shaikh S, Shi H, Stacey S, Croix C, Wang C, Wang Q, Watts A, Gu L, Lin Y, Rabinovitch P, Sweetwyne M, Artyomov M, Ballentine S, Chheda M, Davies S, DiPersio J, Fields R, Fitzpatrick J, Fulton R, Imai S, Jain S, Ju T, Kushnir V, Link D, Ben Major M, Oh S, Rapp D, Rettig M, Stewart S, Veis D, Vij K, Wendl M, Wyczalkowski M, Craft J, Enninful A, Farzad N, Gershkovich P, Halene S, Kluger Y, VanOudenhove J, Xu M, Yang J, Yang M. NIH SenNet Consortium to map senescent cells throughout the human lifespan to understand physiological health Nature Aging 2022, 2: 1090-1100. DOI: 10.1038/s43587-022-00326-5.
Prognostic implications of mono-hit and multi-hit TP53 alterations in patients with acute myeloid leukemia and higher risk myelodysplastic syndromes treated with azacitidine-based therapy
Zeidan A, Bewersdorf J, Hasle V, Shallis R, Thompson E, de Menezes D, Rose S, Boss I, Halene S, Haferlach T, Fox B. Prognostic implications of mono-hit and multi-hit TP53 alterations in patients with acute myeloid leukemia and higher risk myelodysplastic syndromes treated with azacitidine-based therapy Leukemia 2022, 1-4. PMID: 36437356, DOI: 10.1038/s41375-022-01766-z.
Deconvolution of in vivo protein-RNA contacts using fractionated eCLIP-seq
Biancon G, Busarello E, Joshi P, Lesch B, Halene S, Tebaldi T. Deconvolution of in vivo protein-RNA contacts using fractionated eCLIP-seq STAR Protocols 2022, 3: 101823. PMCID: PMC9676202, DOI: 10.1016/j.xpro.2022.101823.
Deconvolution of in vivo protein-RNA contacts using fractionated eCLIP-seq
Biancon G, Busarello E, Joshi P, Lesch B, Halene S, Tebaldi T. Deconvolution of in vivo protein-RNA contacts using fractionated eCLIP-seq STAR Protocols 2022, 3: 101823. PMID: 36595959, PMCID: PMC9676202, DOI: 10.1016/j.xpro.2022.101823.
A Multi-Center Phase Ib Trial of the Histone Deactylase Inhibitor (HDACi) Entinostat in Combination with Anti-PD1 Antibody Pembrolizumab in Patients with Refractory/Relapsed Myelodysplastic Syndromes (RR-MDS) or Oligoblastic Acute Myeloid Leukemia (RR-AML) after Hypomethylating Agent (HMA) Failure
Bewersdorf J, Shallis R, Sharon E, Caldwell A, Wei W, Yacoub A, Madanat Y, Zeidner J, Altman J, Odenike O, Yerrabothala S, Kovacsovics T, Podoltsev N, Halene S, Little R, Piekarz R, Gore S, Kim T, Zeidan A. A Multi-Center Phase Ib Trial of the Histone Deactylase Inhibitor (HDACi) Entinostat in Combination with Anti-PD1 Antibody Pembrolizumab in Patients with Refractory/Relapsed Myelodysplastic Syndromes (RR-MDS) or Oligoblastic Acute Myeloid Leukemia (RR-AML) after Hypomethylating Agent (HMA) Failure Blood 2022, 140: 9084-9086. DOI: 10.1182/blood-2022-158626.
Enhanced Global Disease Assessment with Advanced Imaging and Targeted Myeloma Lesion Biopsy Highlights Spatial Heterogeneity and Detects Residual Disease in Multiple Myeloma
Browning S, Liu Y, Parker T, Bar N, Anderson T, Dhodapkar M, Seropian S, Halene S, Xu M, Syam R, Gorshein E, DiAdamo A, Talsania A, Kidwai W, Witt D, Chang V, Montanari F, Lischuk A, Wei W, Haims A, Neparidze N. Enhanced Global Disease Assessment with Advanced Imaging and Targeted Myeloma Lesion Biopsy Highlights Spatial Heterogeneity and Detects Residual Disease in Multiple Myeloma Blood 2022, 140: 7308-7310. DOI: 10.1182/blood-2022-166325.
Genomic Sequencing of Paired Bone Marrow and Focal Lesion Biopsies of Newly Diagnosed Multiple Myeloma Patients before and after Induction Therapy
Liu Y, Browning S, VanOudenhove J, Biancon G, Bar N, Parker T, Anderson T, Dhodapkar M, Seropian S, Halene S, Xu M, Gorshein E, Talsania A, Kidwai W, Witt D, Chang V, Montanari F, Lischuk A, Haims A, Wei W, Neparidze N. Genomic Sequencing of Paired Bone Marrow and Focal Lesion Biopsies of Newly Diagnosed Multiple Myeloma Patients before and after Induction Therapy Blood 2022, 140: 7086-7087. DOI: 10.1182/blood-2022-168109.
U2AF1 Splicing Factor Mutations Affect RNA Subcellular Compartmentalization in Myeloid Malignancies
Biancon G, Busarello E, Lessard M, Courvan E, Escayola D, VanOudenhove J, Lee V, Krick M, Bewersdorf J, Neugebauer K, Tebaldi T, Halene S. U2AF1 Splicing Factor Mutations Affect RNA Subcellular Compartmentalization in Myeloid Malignancies Blood 2022, 140: 1271-1272. DOI: 10.1182/blood-2022-170043.
Molecular, Epigenetic, and Immune Landscape of TP53- mutated (TP53-M) Acute Myeloid Leukemia (AML) and Higher Risk Myelodysplastic Syndromes (HR-MDS)
Bewersdorf J, Hasle V, Shallis R, Thompson E, Lopes de Menezes D, Rose S, Boss I, Halene S, Haferlach T, Fox B, Zeidan A. Molecular, Epigenetic, and Immune Landscape of TP53- mutated (TP53-M) Acute Myeloid Leukemia (AML) and Higher Risk Myelodysplastic Syndromes (HR-MDS) Blood 2022, 140: 6247-6249. DOI: 10.1182/blood-2022-156460.
Impact of Allelic State on Overall Survival in TP53- mutant Acute Myeloid Leukemia (AML) and Higher Risk Myelodysplastic Syndromes (HR-MDS)
Bewersdorf J, Hasle V, Shallis R, Thompson E, Lopes de Menezes D, Rose S, Boss I, Halene S, Haferlach T, Fox B, Zeidan A. Impact of Allelic State on Overall Survival in TP53- mutant Acute Myeloid Leukemia (AML) and Higher Risk Myelodysplastic Syndromes (HR-MDS) Blood 2022, 140: 8910-8911. DOI: 10.1182/blood-2022-158592.
Harmonizing the Annotation of Hematopoietic Populations in Single-Cell Atlases with the Cell Marker Accordion
Busarello E, Ibnat Z, Biancon G, VanOudenhove J, Lauria F, Viero G, Halene S, Tebaldi T. Harmonizing the Annotation of Hematopoietic Populations in Single-Cell Atlases with the Cell Marker Accordion Blood 2022, 140: 4968-4969. DOI: 10.1182/blood-2022-162180.
Proteomic Profiles of Cytokine Release Syndromes Following Lisocabtagene Maraleucel and Idecabtagene Vicleucel
Mirza A, Pine A, Rasheed Y, Hamouche R, Leveille E, Goshua G, Gu S, Liu Y, VanOudenhove J, Bar N, Neparidze N, Foss F, Gowda L, Isufi I, Halene S, Lee A, Seropian S. Proteomic Profiles of Cytokine Release Syndromes Following Lisocabtagene Maraleucel and Idecabtagene Vicleucel Blood 2022, 140: 10410-10412. DOI: 10.1182/blood-2022-168374.
Repressive β-Catenin-Ikaros Complexes Are Essential to Prevent Clonal Evolution of Human Lymphopoiesis
Cosgun K, Liu W, Robinson M, Fonseca Arce D, VanOudenhove J, Bigogno A, Flavell R, Halene S, Müschen M. Repressive β-Catenin-Ikaros Complexes Are Essential to Prevent Clonal Evolution of Human Lymphopoiesis Blood 2022, 140: 8744-8745. DOI: 10.1182/blood-2022-170409.
Widespread association of the Argonaute protein AGO2 with meiotic chromatin suggests a distinct nuclear function in mammalian male reproduction.
Griffin KN, Walters BW, Li H, Wang H, Biancon G, Tebaldi T, Kaya CB, Kanyo J, Lam TT, Cox AL, Halene S, Chung JJ, Lesch BJ. Widespread association of the Argonaute protein AGO2 with meiotic chromatin suggests a distinct nuclear function in mammalian male reproduction. Genome Research 2022, 32: 1655-1668. PMID: 36109149, PMCID: PMC9528986, DOI: 10.1101/gr.276578.122.
Selective inhibition of MCL1 overcomes venetoclax-resistance in a murine model of myelodysplastic syndromes.
Fischer MA, Song Y, Arrate MP, Gbyli R, Villaume MT, Smith BN, Childress MA, Stricker TP, Halene S, Savona MR. Selective inhibition of MCL1 overcomes venetoclax-resistance in a murine model of myelodysplastic syndromes. Haematologica 2022 PMID: 35979721, DOI: 10.3324/haematol.2022.280631.
Spatial profiling of chromatin accessibility in mouse and human tissues
Deng Y, Bartosovic M, Ma S, Zhang D, Kukanja P, Xiao Y, Su G, Liu Y, Qin X, Rosoklija GB, Dwork AJ, Mann JJ, Xu ML, Halene S, Craft JE, Leong KW, Boldrini M, Castelo-Branco G, Fan R. Spatial profiling of chromatin accessibility in mouse and human tissues Nature 2022, 609: 375-383. PMID: 35978191, PMCID: PMC9452302, DOI: 10.1038/s41586-022-05094-1.
Pre-mRNA splicing factor U2AF2 recognizes distinct conformations of nucleotide variants at the center of the pre-mRNA splice site signal
Glasser E, Maji D, Biancon G, Puthenpeedikakkal AMK, Cavender CE, Tebaldi T, Jenkins JL, Mathews DH, Halene S, Kielkopf CL. Pre-mRNA splicing factor U2AF2 recognizes distinct conformations of nucleotide variants at the center of the pre-mRNA splice site signal Nucleic Acids Research 2022, 50: 5299-5312. PMID: 35524551, PMCID: PMC9128377, DOI: 10.1093/nar/gkac287.
Inflammasome activation in infected macrophages drives COVID-19 pathology
Sefik E, Qu R, Junqueira C, Kaffe E, Mirza H, Zhao J, Brewer JR, Han A, Steach HR, Israelow B, Blackburn HN, Velazquez SE, Chen YG, Halene S, Iwasaki A, Meffre E, Nussenzweig M, Lieberman J, Wilen CB, Kluger Y, Flavell RA. Inflammasome activation in infected macrophages drives COVID-19 pathology Nature 2022, 606: 585-593. PMID: 35483404, PMCID: PMC9288243, DOI: 10.1038/s41586-022-04802-1.
The Coming of Age of Preclinical Models of MDS
Liu W, Teodorescu P, Halene S, Ghiaur G. The Coming of Age of Preclinical Models of MDS Frontiers In Oncology 2022, 12: 815037. PMID: 35372085, PMCID: PMC8966105, DOI: 10.3389/fonc.2022.815037.
In vivo anti-tumor effect of PARP inhibition in IDH1/2 mutant MDS/AML resistant to targeted inhibitors of mutant IDH1/2
Gbyli R, Song Y, Liu W, Gao Y, Biancon G, Chandhok NS, Wang X, Fu X, Patel A, Sundaram R, Tebaldi T, Mamillapalli P, Zeidan AM, Flavell RA, Prebet T, Bindra RS, Halene S. In vivo anti-tumor effect of PARP inhibition in IDH1/2 mutant MDS/AML resistant to targeted inhibitors of mutant IDH1/2 Leukemia 2022, 36: 1313-1323. PMID: 35273342, PMCID: PMC9103411, DOI: 10.1038/s41375-022-01536-x.
Precision analysis of mutant U2AF1 activity reveals deployment of stress granules in myeloid malignancies
Biancon G, Joshi P, Zimmer JT, Hunck T, Gao Y, Lessard MD, Courchaine E, Barentine AES, Machyna M, Botti V, Qin A, Gbyli R, Patel A, Song Y, Kiefer L, Viero G, Neuenkirchen N, Lin H, Bewersdorf J, Simon MD, Neugebauer KM, Tebaldi T, Halene S. Precision analysis of mutant U2AF1 activity reveals deployment of stress granules in myeloid malignancies Molecular Cell 2022, 82: 1107-1122.e7. PMID: 35303483, PMCID: PMC8988922, DOI: 10.1016/j.molcel.2022.02.025.
MYOCARDIAL IMPACT OF CLONAL HEMATOPOIESIS OF INDETERMINATE POTENTIAL (CHIP) SECRETOMES IN CARDIO-ONCOLOGY PATIENTS
Kwan J, Vanoudenhove J, Vanoudenhove J, Halder S, Halder S, Biancon G, Jain K, Jain K, Chakraborty R, Lustberg M, Lustberg M, Pusztai L, Campbell S, Campbell S, Zhao H, Halene S, Hwa J. MYOCARDIAL IMPACT OF CLONAL HEMATOPOIESIS OF INDETERMINATE POTENTIAL (CHIP) SECRETOMES IN CARDIO-ONCOLOGY PATIENTS Journal Of The American College Of Cardiology 2022, 79: 1912. DOI: 10.1016/s0735-1097(22)02903-5.
DNMT3A R882 Mutations Confer Unique Clinicopathologic Features in MDS Including a High Risk of AML Transformation
Jawad M, Afkhami M, Ding Y, Zhang X, Li P, Young K, Xu ML, Cui W, Zhao Y, Halene S, Al-Kali A, Viswanatha D, Chen D, He R, Zheng G. DNMT3A R882 Mutations Confer Unique Clinicopathologic Features in MDS Including a High Risk of AML Transformation Frontiers In Oncology 2022, 12: 849376. PMID: 35296003, PMCID: PMC8918526, DOI: 10.3389/fonc.2022.849376.
A humanized mouse model of chronic COVID-19
Sefik E, Israelow B, Mirza H, Zhao J, Qu R, Kaffe E, Song E, Halene S, Meffre E, Kluger Y, Nussenzweig M, Wilen CB, Iwasaki A, Flavell RA. A humanized mouse model of chronic COVID-19 Nature Biotechnology 2021, 40: 906-920. PMID: 34921308, PMCID: PMC9203605, DOI: 10.1038/s41587-021-01155-4.
ALKBH5 Modulates Hematopoietic Stem and Progenitor Cell Energy Metabolism through m 6a Modification-Mediated RNA Stability
Gao Y, Zimmer J, Vasic R, Liu C, Gbyli R, Zheng S, Patel A, LIU W, Nelakanti R, Song Y, Biancon G, Xiao A, Slavoff S, Simon M, Flavell R, Tebaldi T, Li H, Halene S. ALKBH5 Modulates Hematopoietic Stem and Progenitor Cell Energy Metabolism through m 6a Modification-Mediated RNA Stability Blood 2021, 138: 298-298. DOI: 10.1182/blood-2021-146049.
U2AF1 Mutations Enhance Stress Granule Response in Myeloid Malignancies
Biancon G, Joshi P, Zimmer J, Hunck T, Gao Y, Lessard M, Courchaine E, Barentine A, Machyna M, Botti V, Qin A, Gbyli R, Patel A, Song Y, Kiefer L, Viero G, Neuenkirchen N, Lin H, Bewersdorf J, Simon M, Neugebauer K, Tebaldi T, Halene S. U2AF1 Mutations Enhance Stress Granule Response in Myeloid Malignancies Blood 2021, 138: 321-321. DOI: 10.1182/blood-2021-149618.
Compromised Host Stem Cell Competitiveness Affords Fanconi Stem Cell Engraftment in C-Kit Mutant Humanized Mice
LIU W, Song Y, Gbyli R, Sefik E, Biancon G, Gao Y, Philbrick W, Patel A, Tebaldi T, Nalepa G, Kupfer G, Flavell R, Halene S. Compromised Host Stem Cell Competitiveness Affords Fanconi Stem Cell Engraftment in C-Kit Mutant Humanized Mice Blood 2021, 138: 1119-1119. DOI: 10.1182/blood-2021-153777.
Comprehensive Clinicopathologic and Molecular Analysis of Mast Cell Leukemia With Associated Hematologic Neoplasm: A Report and In-Depth Study of 5 Cases
Li P, Biancon G, Patel T, Pan Z, Kothari S, Halene S, Prebet T, Xu ML. Comprehensive Clinicopathologic and Molecular Analysis of Mast Cell Leukemia With Associated Hematologic Neoplasm: A Report and In-Depth Study of 5 Cases Frontiers In Oncology 2021, 11: 730503. PMID: 34589432, PMCID: PMC8474637, DOI: 10.3389/fonc.2021.730503.
CARDIOVASCULAR AND MORTALITY OUTCOMES IN ONCOLOGY PATIENTS HOSPITALIZED WITH COVID-19
Kwan J, Lee S, Tao W, Wei W, Simonov M, Halene S, Hwa J, Baldassarre L. CARDIOVASCULAR AND MORTALITY OUTCOMES IN ONCOLOGY PATIENTS HOSPITALIZED WITH COVID-19 Journal Of The American College Of Cardiology 2021, 77: 3294. PMCID: PMC8091382, DOI: 10.1016/s0735-1097(21)04648-9.
CARDIOVASCULAR AND MORTALITY OUTCOMES OF HOSPITALIZED COVID-19 PATIENTS STRATIFIED BY RACE AND ETHNICITY
Kwan J, Lee S, Tao W, Wei W, Simonov M, Halene S, Hwa J, Spatz E. CARDIOVASCULAR AND MORTALITY OUTCOMES OF HOSPITALIZED COVID-19 PATIENTS STRATIFIED BY RACE AND ETHNICITY Journal Of The American College Of Cardiology 2021, 77: 3050. PMCID: PMC8091439, DOI: 10.1016/s0735-1097(21)04405-3.
Transcriptome-wide quantification of double-stranded RNAs in live mouse tissues by dsRIP-Seq
Gao Y, Chen S, Halene S, Tebaldi T. Transcriptome-wide quantification of double-stranded RNAs in live mouse tissues by dsRIP-Seq STAR Protocols 2021, 2: 100366. PMID: 33778776, PMCID: PMC7982789, DOI: 10.1016/j.xpro.2021.100366.
Combined liver–cytokine humanization comes to the rescue of circulating human red blood cells
Song Y, Shan L, Gbyli R, Liu W, Strowig T, Patel A, Fu X, Wang X, Xu ML, Gao Y, Qin A, Bruscia EM, Tebaldi T, Biancon G, Mamillapalli P, Urbonas D, Eynon E, Gonzalez DG, Chen J, Krause DS, Alderman J, Halene S, Flavell RA. Combined liver–cytokine humanization comes to the rescue of circulating human red blood cells Science 2021, 371: 1019-1025. PMID: 33674488, PMCID: PMC8292008, DOI: 10.1126/science.abe2485.
A neutrophil activation signature predicts critical illness and mortality in COVID-19
Meizlish ML, Pine AB, Bishai JD, Goshua G, Nadelmann ER, Simonov M, Chang CH, Zhang H, Shallow M, Bahel P, Owusu K, Yamamoto Y, Arora T, Atri DS, Patel A, Gbyli R, Kwan J, Won CH, Dela Cruz C, Price C, Koff J, King BA, Rinder HM, Wilson FP, Hwa J, Halene S, Damsky W, van Dijk D, Lee AI, Chun HJ. A neutrophil activation signature predicts critical illness and mortality in COVID-19 Blood Advances 2021, 5: 1164-1177. PMID: 33635335, PMCID: PMC7908851, DOI: 10.1182/bloodadvances.2020003568.
Editorial: Do not lose focus on myeloid biology in the era of COVID-19.
Halene S, Prebet T. Editorial: Do not lose focus on myeloid biology in the era of COVID-19. Current Opinion In Hematology 2020, 28: 71-72. PMID: 33394725, DOI: 10.1097/moh.0000000000000635.
Thrombocytopathy and endotheliopathy: crucial contributors to COVID-19 thromboinflammation
Gu SX, Tyagi T, Jain K, Gu VW, Lee SH, Hwa JM, Kwan JM, Krause DS, Lee AI, Halene S, Martin KA, Chun HJ, Hwa J. Thrombocytopathy and endotheliopathy: crucial contributors to COVID-19 thromboinflammation Nature Reviews Cardiology 2020, 18: 194-209. PMID: 33214651, PMCID: PMC7675396, DOI: 10.1038/s41569-020-00469-1.
High-Spatial-Resolution Multi-Omics Sequencing via Deterministic Barcoding in Tissue
Liu Y, Yang M, Deng Y, Su G, Enninful A, Guo CC, Tebaldi T, Zhang D, Kim D, Bai Z, Norris E, Pan A, Li J, Xiao Y, Halene S, Fan R. High-Spatial-Resolution Multi-Omics Sequencing via Deterministic Barcoding in Tissue Cell 2020, 183: 1665-1681.e18. PMID: 33188776, PMCID: PMC7736559, DOI: 10.1016/j.cell.2020.10.026.
Advanced Imaging and Targeted Myeloma Lesion Biopsies to Enhance Global Response Assessment and Evaluate Spatial Heterogeneity in Multiple Myeloma
Browning S, Parker T, Bar N, Anderson T, Dhodapkar M, Seropian S, Lee A, Prebet T, Halene S, Xu M, Gorshein E, Talsania A, Kidwai W, Witt D, Chang V, Lischuk A, Wei W, Haims A, Neparidze N. Advanced Imaging and Targeted Myeloma Lesion Biopsies to Enhance Global Response Assessment and Evaluate Spatial Heterogeneity in Multiple Myeloma Blood 2020, 136: 20-22. DOI: 10.1182/blood-2020-136478.
Reconstruction of Sickle Cell Disease with Circulating Sickling Red Blood Cells in Novel Humanized Cytokines and Liver Mistrg Mice
Song Y, Gbyli R, Shan L, Liu W, Gao Y, Patel A, Fu X, Wang X, Xu M, Qin A, Bruscia E, Tebaldi T, Biancon G, Mamillapalli P, Urbonas D, Gonzales D, Krause D, Alderman J, Flavell R, Halene S. Reconstruction of Sickle Cell Disease with Circulating Sickling Red Blood Cells in Novel Humanized Cytokines and Liver Mistrg Mice Blood 2020, 136: 29-30. DOI: 10.1182/blood-2020-141603.
High-Resolution Binding Atlas of U2AF1 Mutants Uncovers New Complexity in Splicing Alterations and Kinetics in Myeloid Malignancies
Biancon G, Joshi P, Hunck T, Zimmer J, Gao Y, Machyna M, Botti V, Qin A, Viero G, Neuenkirchen N, Taylor A, Gbyli R, Patel A, Ardasheva A, Fu X, Lin H, Simon M, Neugebauer K, Tebaldi T, Halene S. High-Resolution Binding Atlas of U2AF1 Mutants Uncovers New Complexity in Splicing Alterations and Kinetics in Myeloid Malignancies Blood 2020, 136: 3-4. DOI: 10.1182/blood-2020-142854.
The Role of RNA Epigenetic Modification in Normal and Malignant Hematopoiesis
Vasic R, Gao Y, Liu C, Halene S. The Role of RNA Epigenetic Modification in Normal and Malignant Hematopoiesis Current Stem Cell Reports 2020, 6: 144-155. PMID: 33777659, PMCID: PMC7992056, DOI: 10.1007/s40778-020-00178-y.
m6A Modification Prevents Formation of Endogenous Double-Stranded RNAs and Deleterious Innate Immune Responses during Hematopoietic Development
Gao Y, Vasic R, Song Y, Teng R, Liu C, Gbyli R, Biancon G, Nelakanti R, Lobben K, Kudo E, Liu W, Ardasheva A, Fu X, Wang X, Joshi P, Lee V, Dura B, Viero G, Iwasaki A, Fan R, Xiao A, Flavell RA, Li HB, Tebaldi T, Halene S. m6A Modification Prevents Formation of Endogenous Double-Stranded RNAs and Deleterious Innate Immune Responses during Hematopoietic Development Immunity 2020, 52: 1007-1021.e8. PMID: 32497523, PMCID: PMC7408742, DOI: 10.1016/j.immuni.2020.05.003.
Humanized mice as preclinical models for myeloid malignancies
Gbyli R, Song Y, Halene S. Humanized mice as preclinical models for myeloid malignancies Biochemical Pharmacology 2020, 174: 113794. PMID: 31926939, DOI: 10.1016/j.bcp.2020.113794.
Functional Analysis of Human Hematopoietic Stem Cells In Vivo in Humanized Mice
Song Y, Gbyli R, Fu X, Halene S. Functional Analysis of Human Hematopoietic Stem Cells In Vivo in Humanized Mice 2019, 2097: 273-289. PMID: 31776933, DOI: 10.1007/978-1-0716-0203-4_18.
Clinical Outcomes of Patients (pts) with TP53-Mutated Acute Myeloid Leukemia (AML) or Myelodysplastic Syndromes (MDS): A Single Center Experience
Bewersdorf J, Shallis R, Gowda L, Hager K, Podoltsev N, Gore S, Prebet T, Halene S, Isufi I, Foss F, Huntington S, Kim T, Pillai M, Parker T, Neparidze N, Bar N, Seropian S, Siddon A, Zeidan A. Clinical Outcomes of Patients (pts) with TP53-Mutated Acute Myeloid Leukemia (AML) or Myelodysplastic Syndromes (MDS): A Single Center Experience Blood 2019, 134: 5173-5173. DOI: 10.1182/blood-2019-127162.
Isolated Trisomy 11 in Patients with Myeloid Malignancies - Is the Prognosis Not As Grim As Previously Thought?
Bewersdorf J, Shallis R, Diadamo A, Gowda L, Podoltsev N, Gore S, Prebet T, Halene S, Isufi I, Foss F, Huntington S, Kim T, Pillai M, Parker T, Neparidze N, Bar N, Seropian S, Siddon A, Zeidan A. Isolated Trisomy 11 in Patients with Myeloid Malignancies - Is the Prognosis Not As Grim As Previously Thought? Blood 2019, 134: 5174-5174. DOI: 10.1182/blood-2019-127277.
Immune Checkpoint Inhibitor Therapy for Acute Myeloid Leukemia and Higher-Risk Myelodysplastic Syndromes: A Single-Center Experience
Shallis R, Bewersdorf J, Gowda L, Podoltsev N, Prebet T, Gore S, Halene S, Isufi I, Foss F, Huntington S, Kim T, Pillai M, Parker T, Neparidze N, Bar N, Seropian S, Zeidan A. Immune Checkpoint Inhibitor Therapy for Acute Myeloid Leukemia and Higher-Risk Myelodysplastic Syndromes: A Single-Center Experience Blood 2019, 134: 1330-1330. DOI: 10.1182/blood-2019-127422.
Loss of METTL3 Mediated m6A RNA Modification Results in Double-Stranded RNA Induced Innate Immune Response and Hematopoietic Failure
Gao Y, Vasic R, Song Y, Teng R, Gbyli R, Biancon G, Nelakanti R, Kudo E, Liu W, Ardasheva A, Fu X, Wang X, Joshi P, Dura B, Lee V, Viero G, Iwasaki A, Fan R, Xiao A, Flavell R, Li H, Tebaldi T, Halene S. Loss of METTL3 Mediated m6A RNA Modification Results in Double-Stranded RNA Induced Innate Immune Response and Hematopoietic Failure Blood 2019, 134: 450-450. DOI: 10.1182/blood-2019-130442.
The PRIME Trial: PARP Inhibition in IDH Mutant Effectiveness Trial. a Phase II Study of Olaparib in Isocitrate Dehydrogenase (IDH) Mutant Relapsed/Refractory Acute Myeloid Leukemia and Myelodysplastic Syndrome
Chandhok N, Wei W, Bindra R, Halene S, Shyr Y, Li J, Berens M, Karlovich C, Ivy S, Prebet T. The PRIME Trial: PARP Inhibition in IDH Mutant Effectiveness Trial. a Phase II Study of Olaparib in Isocitrate Dehydrogenase (IDH) Mutant Relapsed/Refractory Acute Myeloid Leukemia and Myelodysplastic Syndrome Blood 2019, 134: 3909-3909. DOI: 10.1182/blood-2019-129168.
Ivo-Nivo: A Phase II Study of the IDH1 Inhibitor Ivosidenib (AG-120) in Combination with the Checkpoint Blockade Inhibitor Nivolumab for Patients with IDH1 Mutated Relapsed/Refractory AML and High Risk MDS
Chandhok N, Wei W, Halene S, Prebet T. Ivo-Nivo: A Phase II Study of the IDH1 Inhibitor Ivosidenib (AG-120) in Combination with the Checkpoint Blockade Inhibitor Nivolumab for Patients with IDH1 Mutated Relapsed/Refractory AML and High Risk MDS Blood 2019, 134: 1374-1374. DOI: 10.1182/blood-2019-130033.
PARP Inhibitors Are Effective in IDH1/2 Mutant MDS and AML Resistant to Targeted IDH Inhibitors
Gbyli R, Song Y, Liu W, Gao Y, Chandhok N, Fu X, Wang X, Patel A, Sundaram R, Tebaldi T, Biancon G, Ardasheva A, Qin A, Sadykov M, Mamillapalli P, Flavell R, Prebet T, Bindra R, Halene S. PARP Inhibitors Are Effective in IDH1/2 Mutant MDS and AML Resistant to Targeted IDH Inhibitors Blood 2019, 134: 4222-4222. DOI: 10.1182/blood-2019-130814.
U2AF1 Driver Mutations in Hematopoietic Disorders Alter but Do Not Abrogate RNA Binding and Enlighten Structural Dependencies of the U2AF-RNA Complex
Biancon G, Joshi P, Hunck T, Gao Y, Botti V, Qin A, Sadykov M, Wang X, Viero G, Neuenkirchen N, Taylor A, Huang J, Ardasheva A, Fu X, Lin H, Pillai M, Kielkopf C, Neugebauer K, Tebaldi T, Halene S. U2AF1 Driver Mutations in Hematopoietic Disorders Alter but Do Not Abrogate RNA Binding and Enlighten Structural Dependencies of the U2AF-RNA Complex Blood 2019, 134: 1230-1230. DOI: 10.1182/blood-2019-130759.
In Vivo reconstruction of Human Erythropoiesis with Circulating Mature Human RBCs in Humanized Liver Mistrg Mice
Song Y, Shan L, Gbyli R, Liu W, Fu X, Wang X, Qin A, Patel A, Gao Y, Tebaldi T, Biancon G, Urbonas D, Alderman J, Halene S, Flavell R. In Vivo reconstruction of Human Erythropoiesis with Circulating Mature Human RBCs in Humanized Liver Mistrg Mice Blood 2019, 134: 338-338. DOI: 10.1182/blood-2019-130701.
The GPR68/BCL2 Axis Remodels Metabolism in AML By Relocating Calcium
He X, Hawkins C, Lawley L, Wunderlich M, Mizukawa B, Zha X, Halene S, Fang J. The GPR68/BCL2 Axis Remodels Metabolism in AML By Relocating Calcium Blood 2019, 134: 2661-2661. DOI: 10.1182/blood-2019-125455.
LAM-003, a Novel Oral Heat Shock Protein 90 Inhibitor for Treatment of Acute Myeloid Leukemia, Including Wild-Type and FMS-like Tyrosine Kinase 3 (FLT3)-Mutant Disease
Beeharry N, Landrette S, Grotzke J, Gayle S, Hernandez M, Halene S, Young P, Miller L, Xu T, Rothberg J, Lichenstein H. LAM-003, a Novel Oral Heat Shock Protein 90 Inhibitor for Treatment of Acute Myeloid Leukemia, Including Wild-Type and FMS-like Tyrosine Kinase 3 (FLT3)-Mutant Disease Blood 2019, 134: 2664-2664. DOI: 10.1182/blood-2019-125770.
Variable Response to BCL2 Inhibition in MDS Is Enhanced across MDS Subtypes with Synergistic Combination of BCL2+MCL1 Inhibition
Fischer M, Arrate M, Childress M, Smith B, Song Y, Gbyli R, Stricker T, Halene S, Savona M. Variable Response to BCL2 Inhibition in MDS Is Enhanced across MDS Subtypes with Synergistic Combination of BCL2+MCL1 Inhibition Blood 2019, 134: 2982-2982. DOI: 10.1182/blood-2019-126578.
A highly efficient and faithful MDS patient-derived xenotransplantation model for pre-clinical studies
Song Y, Rongvaux A, Taylor A, Jiang T, Tebaldi T, Balasubramanian K, Bagale A, Terzi YK, Gbyli R, Wang X, Fu X, Gao Y, Zhao J, Podoltsev N, Xu M, Neparidze N, Wong E, Torres R, Bruscia EM, Kluger Y, Manz MG, Flavell RA, Halene S. A highly efficient and faithful MDS patient-derived xenotransplantation model for pre-clinical studies Nature Communications 2019, 10: 366. PMID: 30664659, PMCID: PMC6341122, DOI: 10.1038/s41467-018-08166-x.
Low Iron Promotes Megakaryocytic Commitment of Megakaryocytic-Erythroid Progenitors in Human and Mice
Xavier-Ferrucio J, Li X, Scanlon V, Zhang P, Ayala-Lopez N, Tebaldi T, Halene S, Finberg K, Krause D. Low Iron Promotes Megakaryocytic Commitment of Megakaryocytic-Erythroid Progenitors in Human and Mice Blood 2018, 132: 2-2. DOI: 10.1182/blood-2018-99-115124.
Mettl3 Mediated m6A Modification Is Essential in Fetal Hematopoiesis
Gao Y, Vasic R, Tebaldi T, Song Y, Teng R, Joshi P, Viero G, Xiao A, Batista P, Li H, Flavell R, Halene S. Mettl3 Mediated m6A Modification Is Essential in Fetal Hematopoiesis Blood 2018, 132: 3825-3825. DOI: 10.1182/blood-2018-99-119699.
Proton Sensor GPR68 Is Essential to Maintain Myeloid Malignancies
He X, Wunderlich M, Mizukawa B, Mulloy J, Feng S, Lawley L, Hawkins C, Fan W, Zhou G, Zha X, Zhang J, Hodge J, Fan D, Halene S, Fang J. Proton Sensor GPR68 Is Essential to Maintain Myeloid Malignancies Blood 2018, 132: 1353-1353. DOI: 10.1182/blood-2018-99-110399.
Whole Exome Sequencing and Extended Thrombophilia Testing in Patients with Venous Thromboembolism
Shevell L, Lee E, Dhodapkar R, Dykas D, Popa A, Ma D, Bar N, Baluha A, Burns A, Ochoa Chaar C, Dupont A, Gu S, Halene S, Luciano R, Marien R, Neparidze N, Parker T, Yao X, Camire R, Ebberink E, Garcia de Frutos P, Gnanasambandan K, Sayeski P, Huntington J, Lentz S, Mertens K, Parish C, Rezaie R, Connors J, Leavitt A, Bale A, Lee A. Whole Exome Sequencing and Extended Thrombophilia Testing in Patients with Venous Thromboembolism Blood 2018, 132: 2506-2506. DOI: 10.1182/blood-2018-99-115529.
Role of alternative splicing in hematopoietic stem cells during development
Gao Y, Vasic R, Halene S. Role of alternative splicing in hematopoietic stem cells during development Stem Cell Investigation 2018, 5: 26-26. PMID: 30221171, PMCID: PMC6131231, DOI: 10.21037/sci.2018.08.02.
SRSF2 mutations drive oncogenesis by activating a global program of aberrant alternative splicing in hematopoietic cells
Liang Y, Tebaldi T, Rejeski K, Joshi P, Stefani G, Taylor A, Song Y, Vasic R, Maziarz J, Balasubramanian K, Ardasheva A, Ding A, Quattrone A, Halene S. SRSF2 mutations drive oncogenesis by activating a global program of aberrant alternative splicing in hematopoietic cells Leukemia 2018, 32: 2659-2671. PMID: 29858584, PMCID: PMC6274620, DOI: 10.1038/s41375-018-0152-7.
Role of RNA Binding Protein RBM15 in m6A RNA Methylation During Megakaryocytic Differentiation
Ayala‐Lopez N, Ross R, Halene S, Limbach P, Krause D. Role of RNA Binding Protein RBM15 in m6A RNA Methylation During Megakaryocytic Differentiation The FASEB Journal 2018, 32: 790.9-790.9. DOI: 10.1096/fasebj.2018.32.1_supplement.790.9.
Production of 2-Hydroxyglutarate by IDH Mutant Malignancies Induces a BRCAness State That Can be Exploited By PARP Inhibitors and Radiation
Corso C, Bindra R, Glazer P, Sulkowski P, Robinson N, Scanlon S, Purshouse K, Bai H, Liu Y, Sundaram R, Hegan D, Fons N, Breuer G, Song Y, Mishra-Gorur K, De Feyter H, de Graaf R, Surovtseva Y, Kachman M, Halene S, Gunel M. Production of 2-Hydroxyglutarate by IDH Mutant Malignancies Induces a BRCAness State That Can be Exploited By PARP Inhibitors and Radiation International Journal Of Radiation Oncology • Biology • Physics 2017, 99: e68. DOI: 10.1016/j.ijrobp.2017.06.754.
Abstract LB-290: Oncometabolites induce a BRCAness state that can be exploited by PARP inhibitors
Sulkowski P, Corso C, Robinson N, Scanlon S, Purshouse K, Bai H, Liu Y, Sundaram R, Hegan D, Fons N, Breuer G, Song Y, Mishra K, Feyter H, Graaf R, Surovtseva Y, Kachman M, Halene S, Gunel M, Glazer P, Bindra R. Abstract LB-290: Oncometabolites induce a BRCAness state that can be exploited by PARP inhibitors Cancer Research 2017, 77: lb-290-lb-290. DOI: 10.1158/1538-7445.am2017-lb-290.
How do messenger RNA splicing alterations drive myelodysplasia?
Joshi P, Halene S, Abdel-Wahab O. How do messenger RNA splicing alterations drive myelodysplasia? Blood 2017, 129: 2465-2470. PMID: 28348147, PMCID: PMC5418633, DOI: 10.1182/blood-2017-02-692715.
2-Hydroxyglutarate produced by neomorphic IDH mutations suppresses homologous recombination and induces PARP inhibitor sensitivity
Sulkowski PL, Corso CD, Robinson ND, Scanlon SE, Purshouse KR, Bai H, Liu Y, Sundaram RK, Hegan DC, Fons NR, Breuer GA, Song Y, Mishra-Gorur K, De Feyter HM, de Graaf RA, Surovtseva YV, Kachman M, Halene S, Günel M, Glazer PM, Bindra RS. 2-Hydroxyglutarate produced by neomorphic IDH mutations suppresses homologous recombination and induces PARP inhibitor sensitivity Science Translational Medicine 2017, 9 PMID: 28148839, PMCID: PMC5435119, DOI: 10.1126/scitranslmed.aal2463.
Pediatric non–Down syndrome acute megakaryoblastic leukemia is characterized by distinct genomic subsets with varying outcomes
de Rooij JD, Branstetter C, Ma J, Li Y, Walsh MP, Cheng J, Obulkasim A, Dang J, Easton J, Verboon LJ, Mulder HL, Zimmermann M, Koss C, Gupta P, Edmonson M, Rusch M, Lim JY, Reinhardt K, Pigazzi M, Song G, Yeoh AE, Shih LY, Liang DC, Halene S, Krause DS, Zhang J, Downing JR, Locatelli F, Reinhardt D, van den Heuvel-Eibrink MM, Zwaan CM, Fornerod M, Gruber TA. Pediatric non–Down syndrome acute megakaryoblastic leukemia is characterized by distinct genomic subsets with varying outcomes Nature Genetics 2017, 49: 451-456. PMID: 28112737, PMCID: PMC5687824, DOI: 10.1038/ng.3772.
Functional Consequences of Spliceosome Mutations
Halene S. Functional Consequences of Spliceosome Mutations Blood 2016, 128: sci-18-sci-18. DOI: 10.1182/blood.v128.22.sci-18.sci-18.
Novel Developments in Leukopenia and Pancytopenia
Onuoha C, Arshad J, Astle J, Xu M, Halene S. Novel Developments in Leukopenia and Pancytopenia Primary Care Clinics In Office Practice 2016, 43: 559-573. PMID: 27866577, DOI: 10.1016/j.pop.2016.07.005.
Peripheral blood CD34+ cells efficiently engraft human cytokine knock-in mice.
Saito Y, Ellegast JM, Rafiei A, Song Y, Kull D, Heikenwalder M, Rongvaux A, Halene S, Flavell RA, Manz MG. Peripheral blood CD34+ cells efficiently engraft human cytokine knock-in mice. Blood 2016, 128: 1829-1833. PMID: 27543436, PMCID: PMC5054696, DOI: 10.1182/blood-2015-10-676452.
Whole Exome Sequencing in Evaluation of Thrombophilia: A Novel 33-Gene Panel
Lee E, Dykas D, Bale A, Cromwell C, Parker T, Halene S, Burns A, Yao X, Lee A. Whole Exome Sequencing in Evaluation of Thrombophilia: A Novel 33-Gene Panel Blood 2015, 126: 3529-3529. DOI: 10.1182/blood.v126.23.3529.3529.
Efficient Engraftment and Disease Replication of Myelodysplastic Syndromes Using a Novel Humanized Mice Model
Song Y, Taylor A, Rongvaux A, Jiang T, Podoltsev N, Xu M, Neparidze N, Torres R, Barbarotta L, Balasubramanian K, Finberg K, Kluger Y, Flavell R, Halene S. Efficient Engraftment and Disease Replication of Myelodysplastic Syndromes Using a Novel Humanized Mice Model Blood 2015, 126: 4100-4100. DOI: 10.1182/blood.v126.23.4100.4100.
Next Generation Sequencing Identifies a Novel Subset of Non-Down Syndrome Acute Megakaryoblastic Leukemia Characterized By Chimeric Transcripts Involving HOX Cluster Genes
de Rooij J, Branstetter C, Ma J, Li Y, Cheng J, Koss C, Easton J, Verboon L, Mulder H, Rusch M, Lim J, Reinhardt K, Yeoh A, Shih L, Liang D, Halene S, Krause D, Zhang J, Downing J, Locatelli F, Reinhardt D, van den Heuvel-Eibrink M, Forenerod M, Zwaan C, Gruber T. Next Generation Sequencing Identifies a Novel Subset of Non-Down Syndrome Acute Megakaryoblastic Leukemia Characterized By Chimeric Transcripts Involving HOX Cluster Genes Blood 2015, 126: 171-171. DOI: 10.1182/blood.v126.23.171.171.
Integrative Genome-Wide Analysis of RNA Binding and Splicing Reveals Complex Loss and Gain of Function Alterations By SRSF2 P95 Mutations in Myelodysplasia
Rejeski K, Liang Y, Tebaldi T, Stefani G, Taylor A, Maziarz J, Song Y, Balasubramanian K, Vasic R, Kapetanovic E, Abdel-Wahab O, Pillai M, Halene S. Integrative Genome-Wide Analysis of RNA Binding and Splicing Reveals Complex Loss and Gain of Function Alterations By SRSF2 P95 Mutations in Myelodysplasia Blood 2015, 126: 141-141. DOI: 10.1182/blood.v126.23.141.141.
Developing a Model of Human Pluripotent to Hematopoietic Stem Cell Development in Mistrg Mice
Astle J, Xiang Y, Rongvaux A, Weibel C, Elizabeth H, Halene S, Park I, Flavell R. Developing a Model of Human Pluripotent to Hematopoietic Stem Cell Development in Mistrg Mice Blood 2015, 126: 4755-4755. DOI: 10.1182/blood.v126.23.4755.4755.
SRSF2 Mutations Contribute to Myelodysplasia by Mutant-Specific Effects on Exon Recognition
Kim E, Ilagan JO, Liang Y, Daubner GM, Lee S, Ramakrishnan A, Li Y, Chung YR, Micol JB, Murphy ME, Cho H, Kim MK, Zebari AS, Aumann S, Park CY, Buonamici S, Smith PG, Deeg HJ, Lobry C, Aifantis I, Modis Y, Allain F, Halene S, Bradley RK, Abdel-Wahab O. SRSF2 Mutations Contribute to Myelodysplasia by Mutant-Specific Effects on Exon Recognition Cancer Cell 2015, 27: 617-630. PMID: 25965569, PMCID: PMC4429920, DOI: 10.1016/j.ccell.2015.04.006.
26 HUMANIZED MICE AFFORD EFFICIENT ENGRAFTMENT AND DISEASE REPLICATION OF MYELODYSPLASTIC SYNDROMES
Song Y, Rongvaux A, Taylor A, Podoltsev N, Xu M, Neparidze N, Torres R, Barbarotta L, Balasubramanian K, Finberg K, Flavell R, Halene S. 26 HUMANIZED MICE AFFORD EFFICIENT ENGRAFTMENT AND DISEASE REPLICATION OF MYELODYSPLASTIC SYNDROMES Leukemia Research 2015, 39: s11-s12. DOI: 10.1016/s0145-2126(15)30027-8.
22 MUTATIONS IN SRSF2 IDENTIFIED IN MYELODYSPLASIA AFFECT RNA BINDING AFFINITY, SPECIFICITY, AND SPLICING
Liang Y, Liang Y, Daubner G, Daubner G, Tebaldi T, Li Y, Li Y, Stefani G, Stefani G, Taylor A, Taylor A, Maziarz J, Maziarz J, Rejeski K, Rejeski K, Vasic R, Vasic R, Modis Y, Modis Y, Allain F, Allain F, Halene S. 22 MUTATIONS IN SRSF2 IDENTIFIED IN MYELODYSPLASIA AFFECT RNA BINDING AFFINITY, SPECIFICITY, AND SPLICING Leukemia Research 2015, 39: s9-s10. DOI: 10.1016/s0145-2126(15)30023-0.
The regulatory role of serum response factor pathway in neutrophil inflammatory response
Taylor A, Halene S. The regulatory role of serum response factor pathway in neutrophil inflammatory response Current Opinion In Hematology 2015, 22: 67-73. PMID: 25402621, PMCID: PMC4374983, DOI: 10.1097/moh.0000000000000099.
Epithelial (E)-Cadherin Is a Novel Regulator of Platelet Function
Krause D, Halene S, Booth C, Jin H, Zou S, Teixeira A, Zhang P. Epithelial (E)-Cadherin Is a Novel Regulator of Platelet Function Blood 2014, 124: 95-95. DOI: 10.1182/blood.v124.21.95.95.
Tmod3 participates in platelet formation and sizing in mouse fetal liver (278.9)
Sui Z, Robeta N, Sanada C, Halene S, Krause D, Fowler V. Tmod3 participates in platelet formation and sizing in mouse fetal liver (278.9) The FASEB Journal 2014, 28 DOI: 10.1096/fasebj.28.1_supplement.278.9.
Development and function of human innate immune cells in a humanized mouse model
Rongvaux A, Willinger T, Martinek J, Strowig T, Gearty SV, Teichmann LL, Saito Y, Marches F, Halene S, Palucka AK, Manz MG, Flavell RA. Development and function of human innate immune cells in a humanized mouse model Nature Biotechnology 2014, 32: 364-372. PMID: 24633240, PMCID: PMC4017589, DOI: 10.1038/nbt.2858.
SRF is required for neutrophil migration in response to inflammation
Taylor A, Tang W, Bruscia EM, Zhang PX, Lin A, Gaines P, Wu D, Halene S. SRF is required for neutrophil migration in response to inflammation Blood 2014, 123: 3027-3036. PMID: 24574460, PMCID: PMC4014845, DOI: 10.1182/blood-2013-06-507582.
Srf Is Required For Neutrophil Migration In Response To Inflammation
Taylor A, Tang W, Bruscia E, Zhang P, Lin A, Gaines P, Vu L, Wu D, Halene S. Srf Is Required For Neutrophil Migration In Response To Inflammation Blood 2013, 122: 319-319. DOI: 10.1182/blood.v122.21.319.319.
P-225 Modeling of myelodysplasia in a humanized immunodeficient mouse model
Halene S, Rongvaux A, Podoltsev N, Kahlon K, Manz M, Flavell R. P-225 Modeling of myelodysplasia in a humanized immunodeficient mouse model Leukemia Research 2013, 37: s124-s125. DOI: 10.1016/s0145-2126(13)70272-8.
Regional variation in treatment costs and survival for elderly patients with myelodysplastic syndromes.
Ma X, Wang R, Long J, Gold H, Halene S, Gross C. Regional variation in treatment costs and survival for elderly patients with myelodysplastic syndromes. Journal Of Clinical Oncology 2012, 30: 6628-6628. DOI: 10.1200/jco.2012.30.15_suppl.6628.
Serum response factor is an essential transcription factor in megakaryocytic maturation
Halene S, Gao Y, Hahn K, Massaro S, Italiano JE, Schulz V, Lin S, Kupfer GM, Krause DS. Serum response factor is an essential transcription factor in megakaryocytic maturation Blood 2010, 116: 1942-1950. PMID: 20525922, PMCID: PMC3173990, DOI: 10.1182/blood-2010-01-261743.
C/EBPε directs granulocytic-vs-monocytic lineage determination and confers chemotactic function via Hlx
Halene S, Gaines P, Sun H, Zibello T, Lin S, Khanna-Gupta A, Williams SC, Perkins A, Krause D, Berliner N. C/EBPε directs granulocytic-vs-monocytic lineage determination and confers chemotactic function via Hlx Experimental Hematology 2009, 38: 90-103.e4. PMID: 19925846, PMCID: PMC2827304, DOI: 10.1016/j.exphem.2009.11.004.
Serum Response Factor Is An Essential Transcription Factor in Megakaryocytic Maturation.
Halene S, Gao Y, Hahn K, Smith E, Lin S, Perkins A, Krause D. Serum Response Factor Is An Essential Transcription Factor in Megakaryocytic Maturation. Blood 2009, 114: 3652-3652. DOI: 10.1182/blood.v114.22.3652.3652.
Transcriptional Regulation of the Nuclear Envelope Lamin B Receptor: New Roles for GA Binding Protein (GABP) in Neutrophil Development.
Garhwal R, Rosmarin A, Halene S, Gaines P. Transcriptional Regulation of the Nuclear Envelope Lamin B Receptor: New Roles for GA Binding Protein (GABP) in Neutrophil Development. Blood 2009, 114: 1464-1464. DOI: 10.1182/blood.v114.22.1464.1464.
Nucleophosmin-1 Interacts with CCAAT Enhancer Binding Protein Alpha (C/EBPα) to Facilitate Granulocyte Maturation: Implications in MDS and AML.
Khanna-Gupta A, Chen J, Silver M, Sun H, Abayasekara N, Halene S, Sportoletti P, Pandolfi P, Berliner N. Nucleophosmin-1 Interacts with CCAAT Enhancer Binding Protein Alpha (C/EBPα) to Facilitate Granulocyte Maturation: Implications in MDS and AML. Blood 2009, 114: 2768-2768. DOI: 10.1182/blood.v114.22.2768.2768.
Comorbidities and survival in a large cohort of patients with newly diagnosed myelodysplastic syndromes
Wang R, Gross CP, Halene S, Ma X. Comorbidities and survival in a large cohort of patients with newly diagnosed myelodysplastic syndromes Leukemia Research 2009, 33: 1594-1598. PMID: 19324411, PMCID: PMC2749891, DOI: 10.1016/j.leukres.2009.02.005.
OTT-MKL1 and MKL1 Inhibit Wnt Signaling.
Halene S, Cheng E, Schulz V, Tuck D, Krause D. OTT-MKL1 and MKL1 Inhibit Wnt Signaling. Blood 2008, 112: 2250-2250. DOI: 10.1182/blood.v112.11.2250.2250.
Generation of a Factor Dependent Myeloid Cell Line from Nucleophosmin-1 Heterozygous (NPM-1+/−) Mouse Bone Marrow as a Model for 5q- MDS
Khanna-Gupta A, Sun H, Chen J, Halene S, Sportoletti P, Pandolfi P, Berliner N. Generation of a Factor Dependent Myeloid Cell Line from Nucleophosmin-1 Heterozygous (NPM-1+/−) Mouse Bone Marrow as a Model for 5q- MDS Blood 2008, 112: 852-852. DOI: 10.1182/blood.v112.11.852.852.
Dynamic Expression of the Lamin B Receptor and Associated Proteins during Myeloid Differentiation: Insight into Mechanisms of Neutrophil Vs. Macrophage Morphogenesis
Gaines P, Gotur D, Halene S, Olins A, Olins D. Dynamic Expression of the Lamin B Receptor and Associated Proteins during Myeloid Differentiation: Insight into Mechanisms of Neutrophil Vs. Macrophage Morphogenesis Blood 2008, 112: 3546-3546. DOI: 10.1182/blood.v112.11.3546.3546.
Identification of Common Molecular Pathways in Granulopoiesis Associated with C/EBP Epsilon and Gfi-1-Deficient Neutrophil Specific Granule Deficiency (SGD).
Khanna-Gupta A, Halene S, Sun H, Dahl R, Boxer L, Berliner N. Identification of Common Molecular Pathways in Granulopoiesis Associated with C/EBP Epsilon and Gfi-1-Deficient Neutrophil Specific Granule Deficiency (SGD). Blood 2006, 108: 1196-1196. DOI: 10.1182/blood.v108.11.1196.1196.
C/EBPε Knockout (KO) EML and PBiIM Cell Lines Replicate the In Vivo Phenotype of the C/EBPε KO Mouse.
Halene S, Gaines P, Zibello T, Williams S, Khanna-Gupta A, Berliner N. C/EBPε Knockout (KO) EML and PBiIM Cell Lines Replicate the In Vivo Phenotype of the C/EBPε KO Mouse. Blood 2005, 106: 2725-2725. DOI: 10.1182/blood.v106.11.2725.2725.
Effects of the negative control region on expression from retroviral LTR
Wang L, Haas D, Halene S, Kohn DB. Effects of the negative control region on expression from retroviral LTR Molecular Therapy 2003, 7: 438-440. PMID: 12727105, DOI: 10.1016/s1525-0016(03)00035-2.
Gene Therapy Using Hematopoietic Stem Cells: Sisyphus Approaches the Crest
Halene S, Kohn D. Gene Therapy Using Hematopoietic Stem Cells: Sisyphus Approaches the Crest Human Gene Therapy 2000, 11: 1259-1267. PMID: 10890736, DOI: 10.1089/10430340050032366.
Improved expression in hematopoietic and lymphoid cells in mice after transplantation of bone marrow transduced with a modified retroviral vector.
Halene S, Wang L, Cooper R, Bockstoce D, Robbins P, Kohn D. Improved expression in hematopoietic and lymphoid cells in mice after transplantation of bone marrow transduced with a modified retroviral vector. Blood 1999, 94: 3349-57. PMID: 10552944, PMCID: PMC9071851, DOI: 10.1182/blood.v94.10.3349.422k05_3349_3357.
Consistent, persistent expression from modified retroviral vectors in murine hematopoietic stem cells
Robbins P, Skelton D, Yu X, Halene S, Leonard E, Kohn D. Consistent, persistent expression from modified retroviral vectors in murine hematopoietic stem cells Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 10182-10187. PMID: 9707621, PMCID: PMC21482, DOI: 10.1073/pnas.95.17.10182.

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Titles

Arthur H and Isabel Bunker Associate Professor of Medicine (Hematology) and Associate Professor of Pathology
Chief, Section of Hematology
Director, DeLuca Center for Innovation in Hematology Research, Yale Cancer Center
Assistant Medical Director CRSL, Yale Cancer Center

Education & Training

Fellow
Yale University School of Medicine (2006)
Resident
Yale-New Haven Hospital (2002)
Postdoctoral Associate
Childrens Hospital Los Angeles (2000)
Dr Med
Eberhard-Karls-Universitat Tubingen (1997)
Resident
Eberhard-Karls-University (1997)
MD
Eberhard-Karls-Universitat Tubingen (1995)

Languages Spoken

English
Deutsch (German)
Italiano (Italian)
Español (Spanish)

Additional Information

Honors & Recognitions

Professional Service

Publications

Editorial: Molecular advances make the day in myeloid diseases
Halene S. Editorial: Molecular advances make the day in myeloid diseases Current Opinion In Hematology 2023, 30: 29. PMID: 36752625, DOI: 10.1097/moh.0000000000000751.
High-plex protein and whole transcriptome co-mapping at cellular resolution with spatial CITE-seq
Liu Y, DiStasio M, Su G, Asashima H, Enninful A, Qin X, Deng Y, Nam J, Gao F, Bordignon P, Cassano M, Tomayko M, Xu M, Halene S, Craft J, Hafler D, Fan R. High-plex protein and whole transcriptome co-mapping at cellular resolution with spatial CITE-seq Nature Biotechnology 2023, 1-5. PMID: 36823353, DOI: 10.1038/s41587-023-01676-0.
Recruited monocytes/macrophages drive pulmonary neutrophilic inflammation and irreversible lung tissue remodeling in cystic fibrosis
Öz H, Cheng E, Di Pietro C, Tebaldi T, Biancon G, Zeiss C, Zhang P, Huang P, Esquibies S, Britto C, Schupp J, Murray T, Halene S, Krause D, Egan M, Bruscia E. Recruited monocytes/macrophages drive pulmonary neutrophilic inflammation and irreversible lung tissue remodeling in cystic fibrosis Cell Reports 2022, 41: 111797. PMID: 36516754, DOI: 10.1016/j.celrep.2022.111797.
NIH SenNet Consortium to map senescent cells throughout the human lifespan to understand physiological health
Lee P, Benz C, Blood P, Börner K, Campisi J, Chen F, Daldrup-Link H, De Jager P, Ding L, Duncan F, Eickelberg O, Fan R, Finkel T, Furman D, Garovic V, Gehlenborg N, Glass C, Heckenbach I, Joseph Z, Katiyar P, Kim S, Königshoff M, Kuchel G, Lee H, Lee J, Ma J, Ma Q, Melov S, Metis K, Mora A, Musi N, Neretti N, Passos J, Rahman I, Rivera-Mulia J, Robson P, Rojas M, Roy A, Scheibye-Knudsen M, Schilling B, Shi P, Silverstein J, Suryadevara V, Xie J, Wang J, Wong A, Niedernhofer L, Wang S, Anvari H, Balough J, Benz C, Bons J, Brenerman B, Evans W, Gerencser A, Gregory H, Hansen M, Justice J, Kapahi P, Murad N, O’Broin A, Pavone M, Powell M, Scott G, Shanes E, Shankaran M, Verdin E, Winer D, Wu F, Adams A, Blood P, Bueckle A, Cao-Berg I, Chen H, Davis M, Filus S, Hao Y, Hartman A, Hasanaj E, Helfer J, Herr B, Joseph Z, Molla G, Mou G, Puerto J, Quardokus E, Ropelewski A, Ruffalo M, Satija R, Schwenk M, Scibek R, Shirey W, Sibilla M, Welling J, Yuan Z, Bonneau R, Christiano A, Izar B, Menon V, Owens D, Phatnani H, Smith C, Suh Y, Teich A, Bekker V, Chan C, Coutavas E, Hartwig M, Ji Z, Nixon A, Dou Z, Rajagopal J, Slavov N, Holmes D, Jurk D, Kirkland J, Lagnado A, Tchkonia T, Abraham K, Dibattista A, Fridell Y, Howcroft T, Jhappan C, Montes V, Prabhudas M, Resat H, Taylor V, Kumar M, Suryadevara V, Cigarroa F, Cohn R, Cortes T, Courtois E, Chuang J, Davé M, Domanskyi S, Enninga E, Eryilmaz G, Espinoza S, Gelfond J, Kirkland J, Kuchel G, Kuo C, Lehman J, Aguayo-Mazzucato C, Meves A, Rani M, Sanders S, Thibodeau A, Tullius S, Ucar D, White B, Wu Q, Xu M, Yamaguchi S, Assarzadegan N, Cho C, Hwang I, Hwang Y, Xi J, Adeyi O, Aliferis C, Bartolomucci A, Dong X, DuFresne-To M, Ikramuddin S, Johnson S, Nelson A, Niedernhofer L, Revelo X, Trevilla-Garcia C, Sedivy J, Thompson E, Robbins P, Wang J, Aird K, Alder J, Beaulieu D, Bueno M, Calyeca J, Chamucero-Millaris J, Chan S, Chung D, Corbett A, Gorbunova V, Gowdy K, Gurkar A, Horowitz J, Hu Q, Kaur G, Khaliullin T, Lafyatis R, Lanna S, Li D, Ma A, Morris A, Muthumalage T, Peters V, Pryhuber G, Reader B, Rosas L, Sembrat J, Shaikh S, Shi H, Stacey S, Croix C, Wang C, Wang Q, Watts A, Gu L, Lin Y, Rabinovitch P, Sweetwyne M, Artyomov M, Ballentine S, Chheda M, Davies S, DiPersio J, Fields R, Fitzpatrick J, Fulton R, Imai S, Jain S, Ju T, Kushnir V, Link D, Ben Major M, Oh S, Rapp D, Rettig M, Stewart S, Veis D, Vij K, Wendl M, Wyczalkowski M, Craft J, Enninful A, Farzad N, Gershkovich P, Halene S, Kluger Y, VanOudenhove J, Xu M, Yang J, Yang M. NIH SenNet Consortium to map senescent cells throughout the human lifespan to understand physiological health Nature Aging 2022, 2: 1090-1100. DOI: 10.1038/s43587-022-00326-5.
Prognostic implications of mono-hit and multi-hit TP53 alterations in patients with acute myeloid leukemia and higher risk myelodysplastic syndromes treated with azacitidine-based therapy
Zeidan A, Bewersdorf J, Hasle V, Shallis R, Thompson E, de Menezes D, Rose S, Boss I, Halene S, Haferlach T, Fox B. Prognostic implications of mono-hit and multi-hit TP53 alterations in patients with acute myeloid leukemia and higher risk myelodysplastic syndromes treated with azacitidine-based therapy Leukemia 2022, 1-4. PMID: 36437356, DOI: 10.1038/s41375-022-01766-z.
Deconvolution of in vivo protein-RNA contacts using fractionated eCLIP-seq
Biancon G, Busarello E, Joshi P, Lesch B, Halene S, Tebaldi T. Deconvolution of in vivo protein-RNA contacts using fractionated eCLIP-seq STAR Protocols 2022, 3: 101823. PMCID: PMC9676202, DOI: 10.1016/j.xpro.2022.101823.
Deconvolution of in vivo protein-RNA contacts using fractionated eCLIP-seq
Biancon G, Busarello E, Joshi P, Lesch B, Halene S, Tebaldi T. Deconvolution of in vivo protein-RNA contacts using fractionated eCLIP-seq STAR Protocols 2022, 3: 101823. PMID: 36595959, PMCID: PMC9676202, DOI: 10.1016/j.xpro.2022.101823.
A Multi-Center Phase Ib Trial of the Histone Deactylase Inhibitor (HDACi) Entinostat in Combination with Anti-PD1 Antibody Pembrolizumab in Patients with Refractory/Relapsed Myelodysplastic Syndromes (RR-MDS) or Oligoblastic Acute Myeloid Leukemia (RR-AML) after Hypomethylating Agent (HMA) Failure
Bewersdorf J, Shallis R, Sharon E, Caldwell A, Wei W, Yacoub A, Madanat Y, Zeidner J, Altman J, Odenike O, Yerrabothala S, Kovacsovics T, Podoltsev N, Halene S, Little R, Piekarz R, Gore S, Kim T, Zeidan A. A Multi-Center Phase Ib Trial of the Histone Deactylase Inhibitor (HDACi) Entinostat in Combination with Anti-PD1 Antibody Pembrolizumab in Patients with Refractory/Relapsed Myelodysplastic Syndromes (RR-MDS) or Oligoblastic Acute Myeloid Leukemia (RR-AML) after Hypomethylating Agent (HMA) Failure Blood 2022, 140: 9084-9086. DOI: 10.1182/blood-2022-158626.
Enhanced Global Disease Assessment with Advanced Imaging and Targeted Myeloma Lesion Biopsy Highlights Spatial Heterogeneity and Detects Residual Disease in Multiple Myeloma
Browning S, Liu Y, Parker T, Bar N, Anderson T, Dhodapkar M, Seropian S, Halene S, Xu M, Syam R, Gorshein E, DiAdamo A, Talsania A, Kidwai W, Witt D, Chang V, Montanari F, Lischuk A, Wei W, Haims A, Neparidze N. Enhanced Global Disease Assessment with Advanced Imaging and Targeted Myeloma Lesion Biopsy Highlights Spatial Heterogeneity and Detects Residual Disease in Multiple Myeloma Blood 2022, 140: 7308-7310. DOI: 10.1182/blood-2022-166325.
Genomic Sequencing of Paired Bone Marrow and Focal Lesion Biopsies of Newly Diagnosed Multiple Myeloma Patients before and after Induction Therapy
Liu Y, Browning S, VanOudenhove J, Biancon G, Bar N, Parker T, Anderson T, Dhodapkar M, Seropian S, Halene S, Xu M, Gorshein E, Talsania A, Kidwai W, Witt D, Chang V, Montanari F, Lischuk A, Haims A, Wei W, Neparidze N. Genomic Sequencing of Paired Bone Marrow and Focal Lesion Biopsies of Newly Diagnosed Multiple Myeloma Patients before and after Induction Therapy Blood 2022, 140: 7086-7087. DOI: 10.1182/blood-2022-168109.
U2AF1 Splicing Factor Mutations Affect RNA Subcellular Compartmentalization in Myeloid Malignancies
Biancon G, Busarello E, Lessard M, Courvan E, Escayola D, VanOudenhove J, Lee V, Krick M, Bewersdorf J, Neugebauer K, Tebaldi T, Halene S. U2AF1 Splicing Factor Mutations Affect RNA Subcellular Compartmentalization in Myeloid Malignancies Blood 2022, 140: 1271-1272. DOI: 10.1182/blood-2022-170043.
Molecular, Epigenetic, and Immune Landscape of TP53- mutated (TP53-M) Acute Myeloid Leukemia (AML) and Higher Risk Myelodysplastic Syndromes (HR-MDS)
Bewersdorf J, Hasle V, Shallis R, Thompson E, Lopes de Menezes D, Rose S, Boss I, Halene S, Haferlach T, Fox B, Zeidan A. Molecular, Epigenetic, and Immune Landscape of TP53- mutated (TP53-M) Acute Myeloid Leukemia (AML) and Higher Risk Myelodysplastic Syndromes (HR-MDS) Blood 2022, 140: 6247-6249. DOI: 10.1182/blood-2022-156460.
Impact of Allelic State on Overall Survival in TP53- mutant Acute Myeloid Leukemia (AML) and Higher Risk Myelodysplastic Syndromes (HR-MDS)
Bewersdorf J, Hasle V, Shallis R, Thompson E, Lopes de Menezes D, Rose S, Boss I, Halene S, Haferlach T, Fox B, Zeidan A. Impact of Allelic State on Overall Survival in TP53- mutant Acute Myeloid Leukemia (AML) and Higher Risk Myelodysplastic Syndromes (HR-MDS) Blood 2022, 140: 8910-8911. DOI: 10.1182/blood-2022-158592.
Harmonizing the Annotation of Hematopoietic Populations in Single-Cell Atlases with the Cell Marker Accordion
Busarello E, Ibnat Z, Biancon G, VanOudenhove J, Lauria F, Viero G, Halene S, Tebaldi T. Harmonizing the Annotation of Hematopoietic Populations in Single-Cell Atlases with the Cell Marker Accordion Blood 2022, 140: 4968-4969. DOI: 10.1182/blood-2022-162180.
Proteomic Profiles of Cytokine Release Syndromes Following Lisocabtagene Maraleucel and Idecabtagene Vicleucel
Mirza A, Pine A, Rasheed Y, Hamouche R, Leveille E, Goshua G, Gu S, Liu Y, VanOudenhove J, Bar N, Neparidze N, Foss F, Gowda L, Isufi I, Halene S, Lee A, Seropian S. Proteomic Profiles of Cytokine Release Syndromes Following Lisocabtagene Maraleucel and Idecabtagene Vicleucel Blood 2022, 140: 10410-10412. DOI: 10.1182/blood-2022-168374.
Repressive β-Catenin-Ikaros Complexes Are Essential to Prevent Clonal Evolution of Human Lymphopoiesis
Cosgun K, Liu W, Robinson M, Fonseca Arce D, VanOudenhove J, Bigogno A, Flavell R, Halene S, Müschen M. Repressive β-Catenin-Ikaros Complexes Are Essential to Prevent Clonal Evolution of Human Lymphopoiesis Blood 2022, 140: 8744-8745. DOI: 10.1182/blood-2022-170409.
Widespread association of the Argonaute protein AGO2 with meiotic chromatin suggests a distinct nuclear function in mammalian male reproduction.
Griffin KN, Walters BW, Li H, Wang H, Biancon G, Tebaldi T, Kaya CB, Kanyo J, Lam TT, Cox AL, Halene S, Chung JJ, Lesch BJ. Widespread association of the Argonaute protein AGO2 with meiotic chromatin suggests a distinct nuclear function in mammalian male reproduction. Genome Research 2022, 32: 1655-1668. PMID: 36109149, PMCID: PMC9528986, DOI: 10.1101/gr.276578.122.
Selective inhibition of MCL1 overcomes venetoclax-resistance in a murine model of myelodysplastic syndromes.
Fischer MA, Song Y, Arrate MP, Gbyli R, Villaume MT, Smith BN, Childress MA, Stricker TP, Halene S, Savona MR. Selective inhibition of MCL1 overcomes venetoclax-resistance in a murine model of myelodysplastic syndromes. Haematologica 2022 PMID: 35979721, DOI: 10.3324/haematol.2022.280631.
Spatial profiling of chromatin accessibility in mouse and human tissues
Deng Y, Bartosovic M, Ma S, Zhang D, Kukanja P, Xiao Y, Su G, Liu Y, Qin X, Rosoklija GB, Dwork AJ, Mann JJ, Xu ML, Halene S, Craft JE, Leong KW, Boldrini M, Castelo-Branco G, Fan R. Spatial profiling of chromatin accessibility in mouse and human tissues Nature 2022, 609: 375-383. PMID: 35978191, PMCID: PMC9452302, DOI: 10.1038/s41586-022-05094-1.
Pre-mRNA splicing factor U2AF2 recognizes distinct conformations of nucleotide variants at the center of the pre-mRNA splice site signal
Glasser E, Maji D, Biancon G, Puthenpeedikakkal AMK, Cavender CE, Tebaldi T, Jenkins JL, Mathews DH, Halene S, Kielkopf CL. Pre-mRNA splicing factor U2AF2 recognizes distinct conformations of nucleotide variants at the center of the pre-mRNA splice site signal Nucleic Acids Research 2022, 50: 5299-5312. PMID: 35524551, PMCID: PMC9128377, DOI: 10.1093/nar/gkac287.
Inflammasome activation in infected macrophages drives COVID-19 pathology
Sefik E, Qu R, Junqueira C, Kaffe E, Mirza H, Zhao J, Brewer JR, Han A, Steach HR, Israelow B, Blackburn HN, Velazquez SE, Chen YG, Halene S, Iwasaki A, Meffre E, Nussenzweig M, Lieberman J, Wilen CB, Kluger Y, Flavell RA. Inflammasome activation in infected macrophages drives COVID-19 pathology Nature 2022, 606: 585-593. PMID: 35483404, PMCID: PMC9288243, DOI: 10.1038/s41586-022-04802-1.
The Coming of Age of Preclinical Models of MDS
Liu W, Teodorescu P, Halene S, Ghiaur G. The Coming of Age of Preclinical Models of MDS Frontiers In Oncology 2022, 12: 815037. PMID: 35372085, PMCID: PMC8966105, DOI: 10.3389/fonc.2022.815037.
In vivo anti-tumor effect of PARP inhibition in IDH1/2 mutant MDS/AML resistant to targeted inhibitors of mutant IDH1/2
Gbyli R, Song Y, Liu W, Gao Y, Biancon G, Chandhok NS, Wang X, Fu X, Patel A, Sundaram R, Tebaldi T, Mamillapalli P, Zeidan AM, Flavell RA, Prebet T, Bindra RS, Halene S. In vivo anti-tumor effect of PARP inhibition in IDH1/2 mutant MDS/AML resistant to targeted inhibitors of mutant IDH1/2 Leukemia 2022, 36: 1313-1323. PMID: 35273342, PMCID: PMC9103411, DOI: 10.1038/s41375-022-01536-x.
Precision analysis of mutant U2AF1 activity reveals deployment of stress granules in myeloid malignancies
Biancon G, Joshi P, Zimmer JT, Hunck T, Gao Y, Lessard MD, Courchaine E, Barentine AES, Machyna M, Botti V, Qin A, Gbyli R, Patel A, Song Y, Kiefer L, Viero G, Neuenkirchen N, Lin H, Bewersdorf J, Simon MD, Neugebauer KM, Tebaldi T, Halene S. Precision analysis of mutant U2AF1 activity reveals deployment of stress granules in myeloid malignancies Molecular Cell 2022, 82: 1107-1122.e7. PMID: 35303483, PMCID: PMC8988922, DOI: 10.1016/j.molcel.2022.02.025.
MYOCARDIAL IMPACT OF CLONAL HEMATOPOIESIS OF INDETERMINATE POTENTIAL (CHIP) SECRETOMES IN CARDIO-ONCOLOGY PATIENTS
Kwan J, Vanoudenhove J, Vanoudenhove J, Halder S, Halder S, Biancon G, Jain K, Jain K, Chakraborty R, Lustberg M, Lustberg M, Pusztai L, Campbell S, Campbell S, Zhao H, Halene S, Hwa J. MYOCARDIAL IMPACT OF CLONAL HEMATOPOIESIS OF INDETERMINATE POTENTIAL (CHIP) SECRETOMES IN CARDIO-ONCOLOGY PATIENTS Journal Of The American College Of Cardiology 2022, 79: 1912. DOI: 10.1016/s0735-1097(22)02903-5.
DNMT3A R882 Mutations Confer Unique Clinicopathologic Features in MDS Including a High Risk of AML Transformation
Jawad M, Afkhami M, Ding Y, Zhang X, Li P, Young K, Xu ML, Cui W, Zhao Y, Halene S, Al-Kali A, Viswanatha D, Chen D, He R, Zheng G. DNMT3A R882 Mutations Confer Unique Clinicopathologic Features in MDS Including a High Risk of AML Transformation Frontiers In Oncology 2022, 12: 849376. PMID: 35296003, PMCID: PMC8918526, DOI: 10.3389/fonc.2022.849376.
A humanized mouse model of chronic COVID-19
Sefik E, Israelow B, Mirza H, Zhao J, Qu R, Kaffe E, Song E, Halene S, Meffre E, Kluger Y, Nussenzweig M, Wilen CB, Iwasaki A, Flavell RA. A humanized mouse model of chronic COVID-19 Nature Biotechnology 2021, 40: 906-920. PMID: 34921308, PMCID: PMC9203605, DOI: 10.1038/s41587-021-01155-4.
ALKBH5 Modulates Hematopoietic Stem and Progenitor Cell Energy Metabolism through m 6a Modification-Mediated RNA Stability
Gao Y, Zimmer J, Vasic R, Liu C, Gbyli R, Zheng S, Patel A, LIU W, Nelakanti R, Song Y, Biancon G, Xiao A, Slavoff S, Simon M, Flavell R, Tebaldi T, Li H, Halene S. ALKBH5 Modulates Hematopoietic Stem and Progenitor Cell Energy Metabolism through m 6a Modification-Mediated RNA Stability Blood 2021, 138: 298-298. DOI: 10.1182/blood-2021-146049.
U2AF1 Mutations Enhance Stress Granule Response in Myeloid Malignancies
Biancon G, Joshi P, Zimmer J, Hunck T, Gao Y, Lessard M, Courchaine E, Barentine A, Machyna M, Botti V, Qin A, Gbyli R, Patel A, Song Y, Kiefer L, Viero G, Neuenkirchen N, Lin H, Bewersdorf J, Simon M, Neugebauer K, Tebaldi T, Halene S. U2AF1 Mutations Enhance Stress Granule Response in Myeloid Malignancies Blood 2021, 138: 321-321. DOI: 10.1182/blood-2021-149618.
Compromised Host Stem Cell Competitiveness Affords Fanconi Stem Cell Engraftment in C-Kit Mutant Humanized Mice
LIU W, Song Y, Gbyli R, Sefik E, Biancon G, Gao Y, Philbrick W, Patel A, Tebaldi T, Nalepa G, Kupfer G, Flavell R, Halene S. Compromised Host Stem Cell Competitiveness Affords Fanconi Stem Cell Engraftment in C-Kit Mutant Humanized Mice Blood 2021, 138: 1119-1119. DOI: 10.1182/blood-2021-153777.
Comprehensive Clinicopathologic and Molecular Analysis of Mast Cell Leukemia With Associated Hematologic Neoplasm: A Report and In-Depth Study of 5 Cases
Li P, Biancon G, Patel T, Pan Z, Kothari S, Halene S, Prebet T, Xu ML. Comprehensive Clinicopathologic and Molecular Analysis of Mast Cell Leukemia With Associated Hematologic Neoplasm: A Report and In-Depth Study of 5 Cases Frontiers In Oncology 2021, 11: 730503. PMID: 34589432, PMCID: PMC8474637, DOI: 10.3389/fonc.2021.730503.
CARDIOVASCULAR AND MORTALITY OUTCOMES IN ONCOLOGY PATIENTS HOSPITALIZED WITH COVID-19
Kwan J, Lee S, Tao W, Wei W, Simonov M, Halene S, Hwa J, Baldassarre L. CARDIOVASCULAR AND MORTALITY OUTCOMES IN ONCOLOGY PATIENTS HOSPITALIZED WITH COVID-19 Journal Of The American College Of Cardiology 2021, 77: 3294. PMCID: PMC8091382, DOI: 10.1016/s0735-1097(21)04648-9.
CARDIOVASCULAR AND MORTALITY OUTCOMES OF HOSPITALIZED COVID-19 PATIENTS STRATIFIED BY RACE AND ETHNICITY
Kwan J, Lee S, Tao W, Wei W, Simonov M, Halene S, Hwa J, Spatz E. CARDIOVASCULAR AND MORTALITY OUTCOMES OF HOSPITALIZED COVID-19 PATIENTS STRATIFIED BY RACE AND ETHNICITY Journal Of The American College Of Cardiology 2021, 77: 3050. PMCID: PMC8091439, DOI: 10.1016/s0735-1097(21)04405-3.
Transcriptome-wide quantification of double-stranded RNAs in live mouse tissues by dsRIP-Seq
Gao Y, Chen S, Halene S, Tebaldi T. Transcriptome-wide quantification of double-stranded RNAs in live mouse tissues by dsRIP-Seq STAR Protocols 2021, 2: 100366. PMID: 33778776, PMCID: PMC7982789, DOI: 10.1016/j.xpro.2021.100366.
Combined liver–cytokine humanization comes to the rescue of circulating human red blood cells
Song Y, Shan L, Gbyli R, Liu W, Strowig T, Patel A, Fu X, Wang X, Xu ML, Gao Y, Qin A, Bruscia EM, Tebaldi T, Biancon G, Mamillapalli P, Urbonas D, Eynon E, Gonzalez DG, Chen J, Krause DS, Alderman J, Halene S, Flavell RA. Combined liver–cytokine humanization comes to the rescue of circulating human red blood cells Science 2021, 371: 1019-1025. PMID: 33674488, PMCID: PMC8292008, DOI: 10.1126/science.abe2485.
A neutrophil activation signature predicts critical illness and mortality in COVID-19
Meizlish ML, Pine AB, Bishai JD, Goshua G, Nadelmann ER, Simonov M, Chang CH, Zhang H, Shallow M, Bahel P, Owusu K, Yamamoto Y, Arora T, Atri DS, Patel A, Gbyli R, Kwan J, Won CH, Dela Cruz C, Price C, Koff J, King BA, Rinder HM, Wilson FP, Hwa J, Halene S, Damsky W, van Dijk D, Lee AI, Chun HJ. A neutrophil activation signature predicts critical illness and mortality in COVID-19 Blood Advances 2021, 5: 1164-1177. PMID: 33635335, PMCID: PMC7908851, DOI: 10.1182/bloodadvances.2020003568.
Editorial: Do not lose focus on myeloid biology in the era of COVID-19.
Halene S, Prebet T. Editorial: Do not lose focus on myeloid biology in the era of COVID-19. Current Opinion In Hematology 2020, 28: 71-72. PMID: 33394725, DOI: 10.1097/moh.0000000000000635.
Thrombocytopathy and endotheliopathy: crucial contributors to COVID-19 thromboinflammation
Gu SX, Tyagi T, Jain K, Gu VW, Lee SH, Hwa JM, Kwan JM, Krause DS, Lee AI, Halene S, Martin KA, Chun HJ, Hwa J. Thrombocytopathy and endotheliopathy: crucial contributors to COVID-19 thromboinflammation Nature Reviews Cardiology 2020, 18: 194-209. PMID: 33214651, PMCID: PMC7675396, DOI: 10.1038/s41569-020-00469-1.
High-Spatial-Resolution Multi-Omics Sequencing via Deterministic Barcoding in Tissue
Liu Y, Yang M, Deng Y, Su G, Enninful A, Guo CC, Tebaldi T, Zhang D, Kim D, Bai Z, Norris E, Pan A, Li J, Xiao Y, Halene S, Fan R. High-Spatial-Resolution Multi-Omics Sequencing via Deterministic Barcoding in Tissue Cell 2020, 183: 1665-1681.e18. PMID: 33188776, PMCID: PMC7736559, DOI: 10.1016/j.cell.2020.10.026.
Advanced Imaging and Targeted Myeloma Lesion Biopsies to Enhance Global Response Assessment and Evaluate Spatial Heterogeneity in Multiple Myeloma
Browning S, Parker T, Bar N, Anderson T, Dhodapkar M, Seropian S, Lee A, Prebet T, Halene S, Xu M, Gorshein E, Talsania A, Kidwai W, Witt D, Chang V, Lischuk A, Wei W, Haims A, Neparidze N. Advanced Imaging and Targeted Myeloma Lesion Biopsies to Enhance Global Response Assessment and Evaluate Spatial Heterogeneity in Multiple Myeloma Blood 2020, 136: 20-22. DOI: 10.1182/blood-2020-136478.
Reconstruction of Sickle Cell Disease with Circulating Sickling Red Blood Cells in Novel Humanized Cytokines and Liver Mistrg Mice
Song Y, Gbyli R, Shan L, Liu W, Gao Y, Patel A, Fu X, Wang X, Xu M, Qin A, Bruscia E, Tebaldi T, Biancon G, Mamillapalli P, Urbonas D, Gonzales D, Krause D, Alderman J, Flavell R, Halene S. Reconstruction of Sickle Cell Disease with Circulating Sickling Red Blood Cells in Novel Humanized Cytokines and Liver Mistrg Mice Blood 2020, 136: 29-30. DOI: 10.1182/blood-2020-141603.
High-Resolution Binding Atlas of U2AF1 Mutants Uncovers New Complexity in Splicing Alterations and Kinetics in Myeloid Malignancies
Biancon G, Joshi P, Hunck T, Zimmer J, Gao Y, Machyna M, Botti V, Qin A, Viero G, Neuenkirchen N, Taylor A, Gbyli R, Patel A, Ardasheva A, Fu X, Lin H, Simon M, Neugebauer K, Tebaldi T, Halene S. High-Resolution Binding Atlas of U2AF1 Mutants Uncovers New Complexity in Splicing Alterations and Kinetics in Myeloid Malignancies Blood 2020, 136: 3-4. DOI: 10.1182/blood-2020-142854.
The Role of RNA Epigenetic Modification in Normal and Malignant Hematopoiesis
Vasic R, Gao Y, Liu C, Halene S. The Role of RNA Epigenetic Modification in Normal and Malignant Hematopoiesis Current Stem Cell Reports 2020, 6: 144-155. PMID: 33777659, PMCID: PMC7992056, DOI: 10.1007/s40778-020-00178-y.
m6A Modification Prevents Formation of Endogenous Double-Stranded RNAs and Deleterious Innate Immune Responses during Hematopoietic Development
Gao Y, Vasic R, Song Y, Teng R, Liu C, Gbyli R, Biancon G, Nelakanti R, Lobben K, Kudo E, Liu W, Ardasheva A, Fu X, Wang X, Joshi P, Lee V, Dura B, Viero G, Iwasaki A, Fan R, Xiao A, Flavell RA, Li HB, Tebaldi T, Halene S. m6A Modification Prevents Formation of Endogenous Double-Stranded RNAs and Deleterious Innate Immune Responses during Hematopoietic Development Immunity 2020, 52: 1007-1021.e8. PMID: 32497523, PMCID: PMC7408742, DOI: 10.1016/j.immuni.2020.05.003.
Humanized mice as preclinical models for myeloid malignancies
Gbyli R, Song Y, Halene S. Humanized mice as preclinical models for myeloid malignancies Biochemical Pharmacology 2020, 174: 113794. PMID: 31926939, DOI: 10.1016/j.bcp.2020.113794.
Functional Analysis of Human Hematopoietic Stem Cells In Vivo in Humanized Mice
Song Y, Gbyli R, Fu X, Halene S. Functional Analysis of Human Hematopoietic Stem Cells In Vivo in Humanized Mice 2019, 2097: 273-289. PMID: 31776933, DOI: 10.1007/978-1-0716-0203-4_18.
Clinical Outcomes of Patients (pts) with TP53-Mutated Acute Myeloid Leukemia (AML) or Myelodysplastic Syndromes (MDS): A Single Center Experience
Bewersdorf J, Shallis R, Gowda L, Hager K, Podoltsev N, Gore S, Prebet T, Halene S, Isufi I, Foss F, Huntington S, Kim T, Pillai M, Parker T, Neparidze N, Bar N, Seropian S, Siddon A, Zeidan A. Clinical Outcomes of Patients (pts) with TP53-Mutated Acute Myeloid Leukemia (AML) or Myelodysplastic Syndromes (MDS): A Single Center Experience Blood 2019, 134: 5173-5173. DOI: 10.1182/blood-2019-127162.
Isolated Trisomy 11 in Patients with Myeloid Malignancies - Is the Prognosis Not As Grim As Previously Thought?
Bewersdorf J, Shallis R, Diadamo A, Gowda L, Podoltsev N, Gore S, Prebet T, Halene S, Isufi I, Foss F, Huntington S, Kim T, Pillai M, Parker T, Neparidze N, Bar N, Seropian S, Siddon A, Zeidan A. Isolated Trisomy 11 in Patients with Myeloid Malignancies - Is the Prognosis Not As Grim As Previously Thought? Blood 2019, 134: 5174-5174. DOI: 10.1182/blood-2019-127277.
Immune Checkpoint Inhibitor Therapy for Acute Myeloid Leukemia and Higher-Risk Myelodysplastic Syndromes: A Single-Center Experience
Shallis R, Bewersdorf J, Gowda L, Podoltsev N, Prebet T, Gore S, Halene S, Isufi I, Foss F, Huntington S, Kim T, Pillai M, Parker T, Neparidze N, Bar N, Seropian S, Zeidan A. Immune Checkpoint Inhibitor Therapy for Acute Myeloid Leukemia and Higher-Risk Myelodysplastic Syndromes: A Single-Center Experience Blood 2019, 134: 1330-1330. DOI: 10.1182/blood-2019-127422.
Loss of METTL3 Mediated m6A RNA Modification Results in Double-Stranded RNA Induced Innate Immune Response and Hematopoietic Failure
Gao Y, Vasic R, Song Y, Teng R, Gbyli R, Biancon G, Nelakanti R, Kudo E, Liu W, Ardasheva A, Fu X, Wang X, Joshi P, Dura B, Lee V, Viero G, Iwasaki A, Fan R, Xiao A, Flavell R, Li H, Tebaldi T, Halene S. Loss of METTL3 Mediated m6A RNA Modification Results in Double-Stranded RNA Induced Innate Immune Response and Hematopoietic Failure Blood 2019, 134: 450-450. DOI: 10.1182/blood-2019-130442.
The PRIME Trial: PARP Inhibition in IDH Mutant Effectiveness Trial. a Phase II Study of Olaparib in Isocitrate Dehydrogenase (IDH) Mutant Relapsed/Refractory Acute Myeloid Leukemia and Myelodysplastic Syndrome
Chandhok N, Wei W, Bindra R, Halene S, Shyr Y, Li J, Berens M, Karlovich C, Ivy S, Prebet T. The PRIME Trial: PARP Inhibition in IDH Mutant Effectiveness Trial. a Phase II Study of Olaparib in Isocitrate Dehydrogenase (IDH) Mutant Relapsed/Refractory Acute Myeloid Leukemia and Myelodysplastic Syndrome Blood 2019, 134: 3909-3909. DOI: 10.1182/blood-2019-129168.
Ivo-Nivo: A Phase II Study of the IDH1 Inhibitor Ivosidenib (AG-120) in Combination with the Checkpoint Blockade Inhibitor Nivolumab for Patients with IDH1 Mutated Relapsed/Refractory AML and High Risk MDS
Chandhok N, Wei W, Halene S, Prebet T. Ivo-Nivo: A Phase II Study of the IDH1 Inhibitor Ivosidenib (AG-120) in Combination with the Checkpoint Blockade Inhibitor Nivolumab for Patients with IDH1 Mutated Relapsed/Refractory AML and High Risk MDS Blood 2019, 134: 1374-1374. DOI: 10.1182/blood-2019-130033.
PARP Inhibitors Are Effective in IDH1/2 Mutant MDS and AML Resistant to Targeted IDH Inhibitors
Gbyli R, Song Y, Liu W, Gao Y, Chandhok N, Fu X, Wang X, Patel A, Sundaram R, Tebaldi T, Biancon G, Ardasheva A, Qin A, Sadykov M, Mamillapalli P, Flavell R, Prebet T, Bindra R, Halene S. PARP Inhibitors Are Effective in IDH1/2 Mutant MDS and AML Resistant to Targeted IDH Inhibitors Blood 2019, 134: 4222-4222. DOI: 10.1182/blood-2019-130814.
U2AF1 Driver Mutations in Hematopoietic Disorders Alter but Do Not Abrogate RNA Binding and Enlighten Structural Dependencies of the U2AF-RNA Complex
Biancon G, Joshi P, Hunck T, Gao Y, Botti V, Qin A, Sadykov M, Wang X, Viero G, Neuenkirchen N, Taylor A, Huang J, Ardasheva A, Fu X, Lin H, Pillai M, Kielkopf C, Neugebauer K, Tebaldi T, Halene S. U2AF1 Driver Mutations in Hematopoietic Disorders Alter but Do Not Abrogate RNA Binding and Enlighten Structural Dependencies of the U2AF-RNA Complex Blood 2019, 134: 1230-1230. DOI: 10.1182/blood-2019-130759.
In Vivo reconstruction of Human Erythropoiesis with Circulating Mature Human RBCs in Humanized Liver Mistrg Mice
Song Y, Shan L, Gbyli R, Liu W, Fu X, Wang X, Qin A, Patel A, Gao Y, Tebaldi T, Biancon G, Urbonas D, Alderman J, Halene S, Flavell R. In Vivo reconstruction of Human Erythropoiesis with Circulating Mature Human RBCs in Humanized Liver Mistrg Mice Blood 2019, 134: 338-338. DOI: 10.1182/blood-2019-130701.
The GPR68/BCL2 Axis Remodels Metabolism in AML By Relocating Calcium
He X, Hawkins C, Lawley L, Wunderlich M, Mizukawa B, Zha X, Halene S, Fang J. The GPR68/BCL2 Axis Remodels Metabolism in AML By Relocating Calcium Blood 2019, 134: 2661-2661. DOI: 10.1182/blood-2019-125455.
LAM-003, a Novel Oral Heat Shock Protein 90 Inhibitor for Treatment of Acute Myeloid Leukemia, Including Wild-Type and FMS-like Tyrosine Kinase 3 (FLT3)-Mutant Disease
Beeharry N, Landrette S, Grotzke J, Gayle S, Hernandez M, Halene S, Young P, Miller L, Xu T, Rothberg J, Lichenstein H. LAM-003, a Novel Oral Heat Shock Protein 90 Inhibitor for Treatment of Acute Myeloid Leukemia, Including Wild-Type and FMS-like Tyrosine Kinase 3 (FLT3)-Mutant Disease Blood 2019, 134: 2664-2664. DOI: 10.1182/blood-2019-125770.
Variable Response to BCL2 Inhibition in MDS Is Enhanced across MDS Subtypes with Synergistic Combination of BCL2+MCL1 Inhibition
Fischer M, Arrate M, Childress M, Smith B, Song Y, Gbyli R, Stricker T, Halene S, Savona M. Variable Response to BCL2 Inhibition in MDS Is Enhanced across MDS Subtypes with Synergistic Combination of BCL2+MCL1 Inhibition Blood 2019, 134: 2982-2982. DOI: 10.1182/blood-2019-126578.
A highly efficient and faithful MDS patient-derived xenotransplantation model for pre-clinical studies
Song Y, Rongvaux A, Taylor A, Jiang T, Tebaldi T, Balasubramanian K, Bagale A, Terzi YK, Gbyli R, Wang X, Fu X, Gao Y, Zhao J, Podoltsev N, Xu M, Neparidze N, Wong E, Torres R, Bruscia EM, Kluger Y, Manz MG, Flavell RA, Halene S. A highly efficient and faithful MDS patient-derived xenotransplantation model for pre-clinical studies Nature Communications 2019, 10: 366. PMID: 30664659, PMCID: PMC6341122, DOI: 10.1038/s41467-018-08166-x.
Low Iron Promotes Megakaryocytic Commitment of Megakaryocytic-Erythroid Progenitors in Human and Mice
Xavier-Ferrucio J, Li X, Scanlon V, Zhang P, Ayala-Lopez N, Tebaldi T, Halene S, Finberg K, Krause D. Low Iron Promotes Megakaryocytic Commitment of Megakaryocytic-Erythroid Progenitors in Human and Mice Blood 2018, 132: 2-2. DOI: 10.1182/blood-2018-99-115124.
Mettl3 Mediated m6A Modification Is Essential in Fetal Hematopoiesis
Gao Y, Vasic R, Tebaldi T, Song Y, Teng R, Joshi P, Viero G, Xiao A, Batista P, Li H, Flavell R, Halene S. Mettl3 Mediated m6A Modification Is Essential in Fetal Hematopoiesis Blood 2018, 132: 3825-3825. DOI: 10.1182/blood-2018-99-119699.
Proton Sensor GPR68 Is Essential to Maintain Myeloid Malignancies
He X, Wunderlich M, Mizukawa B, Mulloy J, Feng S, Lawley L, Hawkins C, Fan W, Zhou G, Zha X, Zhang J, Hodge J, Fan D, Halene S, Fang J. Proton Sensor GPR68 Is Essential to Maintain Myeloid Malignancies Blood 2018, 132: 1353-1353. DOI: 10.1182/blood-2018-99-110399.
Whole Exome Sequencing and Extended Thrombophilia Testing in Patients with Venous Thromboembolism
Shevell L, Lee E, Dhodapkar R, Dykas D, Popa A, Ma D, Bar N, Baluha A, Burns A, Ochoa Chaar C, Dupont A, Gu S, Halene S, Luciano R, Marien R, Neparidze N, Parker T, Yao X, Camire R, Ebberink E, Garcia de Frutos P, Gnanasambandan K, Sayeski P, Huntington J, Lentz S, Mertens K, Parish C, Rezaie R, Connors J, Leavitt A, Bale A, Lee A. Whole Exome Sequencing and Extended Thrombophilia Testing in Patients with Venous Thromboembolism Blood 2018, 132: 2506-2506. DOI: 10.1182/blood-2018-99-115529.
Role of alternative splicing in hematopoietic stem cells during development
Gao Y, Vasic R, Halene S. Role of alternative splicing in hematopoietic stem cells during development Stem Cell Investigation 2018, 5: 26-26. PMID: 30221171, PMCID: PMC6131231, DOI: 10.21037/sci.2018.08.02.
SRSF2 mutations drive oncogenesis by activating a global program of aberrant alternative splicing in hematopoietic cells
Liang Y, Tebaldi T, Rejeski K, Joshi P, Stefani G, Taylor A, Song Y, Vasic R, Maziarz J, Balasubramanian K, Ardasheva A, Ding A, Quattrone A, Halene S. SRSF2 mutations drive oncogenesis by activating a global program of aberrant alternative splicing in hematopoietic cells Leukemia 2018, 32: 2659-2671. PMID: 29858584, PMCID: PMC6274620, DOI: 10.1038/s41375-018-0152-7.
Role of RNA Binding Protein RBM15 in m6A RNA Methylation During Megakaryocytic Differentiation
Ayala‐Lopez N, Ross R, Halene S, Limbach P, Krause D. Role of RNA Binding Protein RBM15 in m6A RNA Methylation During Megakaryocytic Differentiation The FASEB Journal 2018, 32: 790.9-790.9. DOI: 10.1096/fasebj.2018.32.1_supplement.790.9.
Production of 2-Hydroxyglutarate by IDH Mutant Malignancies Induces a BRCAness State That Can be Exploited By PARP Inhibitors and Radiation
Corso C, Bindra R, Glazer P, Sulkowski P, Robinson N, Scanlon S, Purshouse K, Bai H, Liu Y, Sundaram R, Hegan D, Fons N, Breuer G, Song Y, Mishra-Gorur K, De Feyter H, de Graaf R, Surovtseva Y, Kachman M, Halene S, Gunel M. Production of 2-Hydroxyglutarate by IDH Mutant Malignancies Induces a BRCAness State That Can be Exploited By PARP Inhibitors and Radiation International Journal Of Radiation Oncology • Biology • Physics 2017, 99: e68. DOI: 10.1016/j.ijrobp.2017.06.754.
Abstract LB-290: Oncometabolites induce a BRCAness state that can be exploited by PARP inhibitors
Sulkowski P, Corso C, Robinson N, Scanlon S, Purshouse K, Bai H, Liu Y, Sundaram R, Hegan D, Fons N, Breuer G, Song Y, Mishra K, Feyter H, Graaf R, Surovtseva Y, Kachman M, Halene S, Gunel M, Glazer P, Bindra R. Abstract LB-290: Oncometabolites induce a BRCAness state that can be exploited by PARP inhibitors Cancer Research 2017, 77: lb-290-lb-290. DOI: 10.1158/1538-7445.am2017-lb-290.
How do messenger RNA splicing alterations drive myelodysplasia?
Joshi P, Halene S, Abdel-Wahab O. How do messenger RNA splicing alterations drive myelodysplasia? Blood 2017, 129: 2465-2470. PMID: 28348147, PMCID: PMC5418633, DOI: 10.1182/blood-2017-02-692715.
2-Hydroxyglutarate produced by neomorphic IDH mutations suppresses homologous recombination and induces PARP inhibitor sensitivity
Sulkowski PL, Corso CD, Robinson ND, Scanlon SE, Purshouse KR, Bai H, Liu Y, Sundaram RK, Hegan DC, Fons NR, Breuer GA, Song Y, Mishra-Gorur K, De Feyter HM, de Graaf RA, Surovtseva YV, Kachman M, Halene S, Günel M, Glazer PM, Bindra RS. 2-Hydroxyglutarate produced by neomorphic IDH mutations suppresses homologous recombination and induces PARP inhibitor sensitivity Science Translational Medicine 2017, 9 PMID: 28148839, PMCID: PMC5435119, DOI: 10.1126/scitranslmed.aal2463.
Pediatric non–Down syndrome acute megakaryoblastic leukemia is characterized by distinct genomic subsets with varying outcomes
de Rooij JD, Branstetter C, Ma J, Li Y, Walsh MP, Cheng J, Obulkasim A, Dang J, Easton J, Verboon LJ, Mulder HL, Zimmermann M, Koss C, Gupta P, Edmonson M, Rusch M, Lim JY, Reinhardt K, Pigazzi M, Song G, Yeoh AE, Shih LY, Liang DC, Halene S, Krause DS, Zhang J, Downing JR, Locatelli F, Reinhardt D, van den Heuvel-Eibrink MM, Zwaan CM, Fornerod M, Gruber TA. Pediatric non–Down syndrome acute megakaryoblastic leukemia is characterized by distinct genomic subsets with varying outcomes Nature Genetics 2017, 49: 451-456. PMID: 28112737, PMCID: PMC5687824, DOI: 10.1038/ng.3772.
Functional Consequences of Spliceosome Mutations
Halene S. Functional Consequences of Spliceosome Mutations Blood 2016, 128: sci-18-sci-18. DOI: 10.1182/blood.v128.22.sci-18.sci-18.
Novel Developments in Leukopenia and Pancytopenia
Onuoha C, Arshad J, Astle J, Xu M, Halene S. Novel Developments in Leukopenia and Pancytopenia Primary Care Clinics In Office Practice 2016, 43: 559-573. PMID: 27866577, DOI: 10.1016/j.pop.2016.07.005.
Peripheral blood CD34+ cells efficiently engraft human cytokine knock-in mice.
Saito Y, Ellegast JM, Rafiei A, Song Y, Kull D, Heikenwalder M, Rongvaux A, Halene S, Flavell RA, Manz MG. Peripheral blood CD34+ cells efficiently engraft human cytokine knock-in mice. Blood 2016, 128: 1829-1833. PMID: 27543436, PMCID: PMC5054696, DOI: 10.1182/blood-2015-10-676452.
Whole Exome Sequencing in Evaluation of Thrombophilia: A Novel 33-Gene Panel
Lee E, Dykas D, Bale A, Cromwell C, Parker T, Halene S, Burns A, Yao X, Lee A. Whole Exome Sequencing in Evaluation of Thrombophilia: A Novel 33-Gene Panel Blood 2015, 126: 3529-3529. DOI: 10.1182/blood.v126.23.3529.3529.
Efficient Engraftment and Disease Replication of Myelodysplastic Syndromes Using a Novel Humanized Mice Model
Song Y, Taylor A, Rongvaux A, Jiang T, Podoltsev N, Xu M, Neparidze N, Torres R, Barbarotta L, Balasubramanian K, Finberg K, Kluger Y, Flavell R, Halene S. Efficient Engraftment and Disease Replication of Myelodysplastic Syndromes Using a Novel Humanized Mice Model Blood 2015, 126: 4100-4100. DOI: 10.1182/blood.v126.23.4100.4100.
Next Generation Sequencing Identifies a Novel Subset of Non-Down Syndrome Acute Megakaryoblastic Leukemia Characterized By Chimeric Transcripts Involving HOX Cluster Genes
de Rooij J, Branstetter C, Ma J, Li Y, Cheng J, Koss C, Easton J, Verboon L, Mulder H, Rusch M, Lim J, Reinhardt K, Yeoh A, Shih L, Liang D, Halene S, Krause D, Zhang J, Downing J, Locatelli F, Reinhardt D, van den Heuvel-Eibrink M, Forenerod M, Zwaan C, Gruber T. Next Generation Sequencing Identifies a Novel Subset of Non-Down Syndrome Acute Megakaryoblastic Leukemia Characterized By Chimeric Transcripts Involving HOX Cluster Genes Blood 2015, 126: 171-171. DOI: 10.1182/blood.v126.23.171.171.
Integrative Genome-Wide Analysis of RNA Binding and Splicing Reveals Complex Loss and Gain of Function Alterations By SRSF2 P95 Mutations in Myelodysplasia
Rejeski K, Liang Y, Tebaldi T, Stefani G, Taylor A, Maziarz J, Song Y, Balasubramanian K, Vasic R, Kapetanovic E, Abdel-Wahab O, Pillai M, Halene S. Integrative Genome-Wide Analysis of RNA Binding and Splicing Reveals Complex Loss and Gain of Function Alterations By SRSF2 P95 Mutations in Myelodysplasia Blood 2015, 126: 141-141. DOI: 10.1182/blood.v126.23.141.141.
Developing a Model of Human Pluripotent to Hematopoietic Stem Cell Development in Mistrg Mice
Astle J, Xiang Y, Rongvaux A, Weibel C, Elizabeth H, Halene S, Park I, Flavell R. Developing a Model of Human Pluripotent to Hematopoietic Stem Cell Development in Mistrg Mice Blood 2015, 126: 4755-4755. DOI: 10.1182/blood.v126.23.4755.4755.
SRSF2 Mutations Contribute to Myelodysplasia by Mutant-Specific Effects on Exon Recognition
Kim E, Ilagan JO, Liang Y, Daubner GM, Lee S, Ramakrishnan A, Li Y, Chung YR, Micol JB, Murphy ME, Cho H, Kim MK, Zebari AS, Aumann S, Park CY, Buonamici S, Smith PG, Deeg HJ, Lobry C, Aifantis I, Modis Y, Allain F, Halene S, Bradley RK, Abdel-Wahab O. SRSF2 Mutations Contribute to Myelodysplasia by Mutant-Specific Effects on Exon Recognition Cancer Cell 2015, 27: 617-630. PMID: 25965569, PMCID: PMC4429920, DOI: 10.1016/j.ccell.2015.04.006.
26 HUMANIZED MICE AFFORD EFFICIENT ENGRAFTMENT AND DISEASE REPLICATION OF MYELODYSPLASTIC SYNDROMES
Song Y, Rongvaux A, Taylor A, Podoltsev N, Xu M, Neparidze N, Torres R, Barbarotta L, Balasubramanian K, Finberg K, Flavell R, Halene S. 26 HUMANIZED MICE AFFORD EFFICIENT ENGRAFTMENT AND DISEASE REPLICATION OF MYELODYSPLASTIC SYNDROMES Leukemia Research 2015, 39: s11-s12. DOI: 10.1016/s0145-2126(15)30027-8.
22 MUTATIONS IN SRSF2 IDENTIFIED IN MYELODYSPLASIA AFFECT RNA BINDING AFFINITY, SPECIFICITY, AND SPLICING
Liang Y, Liang Y, Daubner G, Daubner G, Tebaldi T, Li Y, Li Y, Stefani G, Stefani G, Taylor A, Taylor A, Maziarz J, Maziarz J, Rejeski K, Rejeski K, Vasic R, Vasic R, Modis Y, Modis Y, Allain F, Allain F, Halene S. 22 MUTATIONS IN SRSF2 IDENTIFIED IN MYELODYSPLASIA AFFECT RNA BINDING AFFINITY, SPECIFICITY, AND SPLICING Leukemia Research 2015, 39: s9-s10. DOI: 10.1016/s0145-2126(15)30023-0.
The regulatory role of serum response factor pathway in neutrophil inflammatory response
Taylor A, Halene S. The regulatory role of serum response factor pathway in neutrophil inflammatory response Current Opinion In Hematology 2015, 22: 67-73. PMID: 25402621, PMCID: PMC4374983, DOI: 10.1097/moh.0000000000000099.
Epithelial (E)-Cadherin Is a Novel Regulator of Platelet Function
Krause D, Halene S, Booth C, Jin H, Zou S, Teixeira A, Zhang P. Epithelial (E)-Cadherin Is a Novel Regulator of Platelet Function Blood 2014, 124: 95-95. DOI: 10.1182/blood.v124.21.95.95.
Tmod3 participates in platelet formation and sizing in mouse fetal liver (278.9)
Sui Z, Robeta N, Sanada C, Halene S, Krause D, Fowler V. Tmod3 participates in platelet formation and sizing in mouse fetal liver (278.9) The FASEB Journal 2014, 28 DOI: 10.1096/fasebj.28.1_supplement.278.9.
Development and function of human innate immune cells in a humanized mouse model
Rongvaux A, Willinger T, Martinek J, Strowig T, Gearty SV, Teichmann LL, Saito Y, Marches F, Halene S, Palucka AK, Manz MG, Flavell RA. Development and function of human innate immune cells in a humanized mouse model Nature Biotechnology 2014, 32: 364-372. PMID: 24633240, PMCID: PMC4017589, DOI: 10.1038/nbt.2858.
SRF is required for neutrophil migration in response to inflammation
Taylor A, Tang W, Bruscia EM, Zhang PX, Lin A, Gaines P, Wu D, Halene S. SRF is required for neutrophil migration in response to inflammation Blood 2014, 123: 3027-3036. PMID: 24574460, PMCID: PMC4014845, DOI: 10.1182/blood-2013-06-507582.
Srf Is Required For Neutrophil Migration In Response To Inflammation
Taylor A, Tang W, Bruscia E, Zhang P, Lin A, Gaines P, Vu L, Wu D, Halene S. Srf Is Required For Neutrophil Migration In Response To Inflammation Blood 2013, 122: 319-319. DOI: 10.1182/blood.v122.21.319.319.
P-225 Modeling of myelodysplasia in a humanized immunodeficient mouse model
Halene S, Rongvaux A, Podoltsev N, Kahlon K, Manz M, Flavell R. P-225 Modeling of myelodysplasia in a humanized immunodeficient mouse model Leukemia Research 2013, 37: s124-s125. DOI: 10.1016/s0145-2126(13)70272-8.
Regional variation in treatment costs and survival for elderly patients with myelodysplastic syndromes.
Ma X, Wang R, Long J, Gold H, Halene S, Gross C. Regional variation in treatment costs and survival for elderly patients with myelodysplastic syndromes. Journal Of Clinical Oncology 2012, 30: 6628-6628. DOI: 10.1200/jco.2012.30.15_suppl.6628.
Serum response factor is an essential transcription factor in megakaryocytic maturation
Halene S, Gao Y, Hahn K, Massaro S, Italiano JE, Schulz V, Lin S, Kupfer GM, Krause DS. Serum response factor is an essential transcription factor in megakaryocytic maturation Blood 2010, 116: 1942-1950. PMID: 20525922, PMCID: PMC3173990, DOI: 10.1182/blood-2010-01-261743.
C/EBPε directs granulocytic-vs-monocytic lineage determination and confers chemotactic function via Hlx
Halene S, Gaines P, Sun H, Zibello T, Lin S, Khanna-Gupta A, Williams SC, Perkins A, Krause D, Berliner N. C/EBPε directs granulocytic-vs-monocytic lineage determination and confers chemotactic function via Hlx Experimental Hematology 2009, 38: 90-103.e4. PMID: 19925846, PMCID: PMC2827304, DOI: 10.1016/j.exphem.2009.11.004.
Serum Response Factor Is An Essential Transcription Factor in Megakaryocytic Maturation.
Halene S, Gao Y, Hahn K, Smith E, Lin S, Perkins A, Krause D. Serum Response Factor Is An Essential Transcription Factor in Megakaryocytic Maturation. Blood 2009, 114: 3652-3652. DOI: 10.1182/blood.v114.22.3652.3652.
Transcriptional Regulation of the Nuclear Envelope Lamin B Receptor: New Roles for GA Binding Protein (GABP) in Neutrophil Development.
Garhwal R, Rosmarin A, Halene S, Gaines P. Transcriptional Regulation of the Nuclear Envelope Lamin B Receptor: New Roles for GA Binding Protein (GABP) in Neutrophil Development. Blood 2009, 114: 1464-1464. DOI: 10.1182/blood.v114.22.1464.1464.
Nucleophosmin-1 Interacts with CCAAT Enhancer Binding Protein Alpha (C/EBPα) to Facilitate Granulocyte Maturation: Implications in MDS and AML.
Khanna-Gupta A, Chen J, Silver M, Sun H, Abayasekara N, Halene S, Sportoletti P, Pandolfi P, Berliner N. Nucleophosmin-1 Interacts with CCAAT Enhancer Binding Protein Alpha (C/EBPα) to Facilitate Granulocyte Maturation: Implications in MDS and AML. Blood 2009, 114: 2768-2768. DOI: 10.1182/blood.v114.22.2768.2768.
Comorbidities and survival in a large cohort of patients with newly diagnosed myelodysplastic syndromes
Wang R, Gross CP, Halene S, Ma X. Comorbidities and survival in a large cohort of patients with newly diagnosed myelodysplastic syndromes Leukemia Research 2009, 33: 1594-1598. PMID: 19324411, PMCID: PMC2749891, DOI: 10.1016/j.leukres.2009.02.005.
OTT-MKL1 and MKL1 Inhibit Wnt Signaling.
Halene S, Cheng E, Schulz V, Tuck D, Krause D. OTT-MKL1 and MKL1 Inhibit Wnt Signaling. Blood 2008, 112: 2250-2250. DOI: 10.1182/blood.v112.11.2250.2250.
Generation of a Factor Dependent Myeloid Cell Line from Nucleophosmin-1 Heterozygous (NPM-1+/−) Mouse Bone Marrow as a Model for 5q- MDS
Khanna-Gupta A, Sun H, Chen J, Halene S, Sportoletti P, Pandolfi P, Berliner N. Generation of a Factor Dependent Myeloid Cell Line from Nucleophosmin-1 Heterozygous (NPM-1+/−) Mouse Bone Marrow as a Model for 5q- MDS Blood 2008, 112: 852-852. DOI: 10.1182/blood.v112.11.852.852.
Dynamic Expression of the Lamin B Receptor and Associated Proteins during Myeloid Differentiation: Insight into Mechanisms of Neutrophil Vs. Macrophage Morphogenesis
Gaines P, Gotur D, Halene S, Olins A, Olins D. Dynamic Expression of the Lamin B Receptor and Associated Proteins during Myeloid Differentiation: Insight into Mechanisms of Neutrophil Vs. Macrophage Morphogenesis Blood 2008, 112: 3546-3546. DOI: 10.1182/blood.v112.11.3546.3546.
Identification of Common Molecular Pathways in Granulopoiesis Associated with C/EBP Epsilon and Gfi-1-Deficient Neutrophil Specific Granule Deficiency (SGD).
Khanna-Gupta A, Halene S, Sun H, Dahl R, Boxer L, Berliner N. Identification of Common Molecular Pathways in Granulopoiesis Associated with C/EBP Epsilon and Gfi-1-Deficient Neutrophil Specific Granule Deficiency (SGD). Blood 2006, 108: 1196-1196. DOI: 10.1182/blood.v108.11.1196.1196.
C/EBPε Knockout (KO) EML and PBiIM Cell Lines Replicate the In Vivo Phenotype of the C/EBPε KO Mouse.
Halene S, Gaines P, Zibello T, Williams S, Khanna-Gupta A, Berliner N. C/EBPε Knockout (KO) EML and PBiIM Cell Lines Replicate the In Vivo Phenotype of the C/EBPε KO Mouse. Blood 2005, 106: 2725-2725. DOI: 10.1182/blood.v106.11.2725.2725.
Effects of the negative control region on expression from retroviral LTR
Wang L, Haas D, Halene S, Kohn DB. Effects of the negative control region on expression from retroviral LTR Molecular Therapy 2003, 7: 438-440. PMID: 12727105, DOI: 10.1016/s1525-0016(03)00035-2.
Gene Therapy Using Hematopoietic Stem Cells: Sisyphus Approaches the Crest
Halene S, Kohn D. Gene Therapy Using Hematopoietic Stem Cells: Sisyphus Approaches the Crest Human Gene Therapy 2000, 11: 1259-1267. PMID: 10890736, DOI: 10.1089/10430340050032366.
Improved expression in hematopoietic and lymphoid cells in mice after transplantation of bone marrow transduced with a modified retroviral vector.
Halene S, Wang L, Cooper R, Bockstoce D, Robbins P, Kohn D. Improved expression in hematopoietic and lymphoid cells in mice after transplantation of bone marrow transduced with a modified retroviral vector. Blood 1999, 94: 3349-57. PMID: 10552944, PMCID: PMC9071851, DOI: 10.1182/blood.v94.10.3349.422k05_3349_3357.
Consistent, persistent expression from modified retroviral vectors in murine hematopoietic stem cells
Robbins P, Skelton D, Yu X, Halene S, Leonard E, Kohn D. Consistent, persistent expression from modified retroviral vectors in murine hematopoietic stem cells Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 10182-10187. PMID: 9707621, PMCID: PMC21482, DOI: 10.1073/pnas.95.17.10182.

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Yale Cancer Center
Yale New Haven Hospital
20 York Street
New Haven, CT 06510
- 1-203-200-4363
- Directions