Deciphering endothelial dysfunction underlying bronchiolitis through integrated multi-omic profiling

The primary objective of this study is to establish that less IL-1 and more endogenous IL-1 inhibitory molecule concentrations are responsible for resolution of hypoxic respiratory failure the population of critically ill children with bronchiolitis in the severity of hypoxemic respiratory failure in children with bronchiolitis.

Study activities will include:

• Collection of secretions from your child’s lungs during routine suctioning
• Collection of data collected as part of clinical care.

This will be done, at maximum, daily until the breathing tube is removed. No additional procedures or fluid is collected as part of this research study. Samples and data collected will be stored in a repository to be used for future research.

Your child will be involved in the study only while admitted to the hospital. Sample collection will occur only while your child has a breathing tube on the ventilator. Once their breathing tube is removed, we will only collect data from the rest of the hospital stay. Data collected during the stay will come from your child’s medical records. Examples of this data include age, sex, laboratory results and medications and interventions that your child received.

We do not expect any risks from taking part in this study. Suctioning secretions out of your child’s lungs is part of their normal and required care while the breathing tube is in place. This procedure is done as part of the standard of care and that we are collecting secretions that would otherwise be discarded.

This research is funded by the National Heart, Lung and Blood Institute of the National Institutes of Health. This study will not perform any DNA genetic sequencing and will not keep or generate any cell lines from your child’s samples. The proposal will perform RNA sequencing on the single cells in your child’s lung fluid. RNA-sequencing provides a measure of how each cell is responding to viral infection and is not identifiable without additional information. No identifiable or protected health information will be used in the analysis of the RNA-sequencing data. After the study is completed, all the data will be anonymously deposited in the National Center for Biotechnology Information’s Gene Expression Omnibus database. No identifying or protected health information will accompany that submission.