My clinical training is as an advanced heart failure and cardiac transplantation physician with formal research training in patient oriented translational research. My research program is supported by NIH and investigator-initiated industry sources and uses techniques of both translational and clinical research to further the mechanistic understanding of cardiac-renal interactions, fluid and sodium homeostasis, and diuretic resistance in heart failure. The primary approach of my current research program is prospective mechanistic human studies in hospitalized heart failure patients, for which we have established extensive infrastructure. Notably, we have enrolled over 700 patients and conducted over 1300 intense biospecimen collections resulting in the over 500,000 biospecimens currently housed in our biorepository at Yale. My lab has extensive expertise in biomarker research has established and maintains an extensive bioassay resource with the capability to determine an extensive range of analytes in human biological samples with complexity ranging from simple serum chemistries to complex analytes such as ultra-trace lithium by inductively coupled plasma mass spectrometry, deuterium concentration by nuclear magnetic resonance, high dimension multiplex protein assays, and large and small molecules by liquid chromatography mass spectrometry.